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  • Featured Product
  • KD/KO Validated

VPS33B Polyclonal antibody

VPS33B Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12195-1-AP

Synonyms

hVPS33B, VPS33B

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 12195-1-AP

    WB analysis of HEK-293 using 12195-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 12195-1-AP

    WB analysis of HEK-293 using 12195-1-AP

    WB result of VPS33B antibody (12195-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS33B transfected HEK-293 cells.

  • WB analysis of mouse testis using 12195-1-AP

    WB analysis of mouse testis using 12195-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 12195-1-AP

    WB analysis of L02 using 12195-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 12195-1-AP

    WB analysis of mouse skeletal muscle using 12195-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 12195-1-AP

    WB analysis of HeLa using 12195-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12195-1-AP (VPS33B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 12195-1-AP

    IHC staining of human colon cancer using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 12195-1-AP

    IHC staining of human colon cancer using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12195-1-AP (VPS33B Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary using 12195-1-AP

    IHC staining of human ovary using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human medulloblastoma using 12195-1-AP

    IHC staining of human medulloblastoma using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12195-1-AP (VPS33B antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 12195-1-AP

    IHC staining of human kidney using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human kidney using 12195-1-AP

    IHC staining of human kidney using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human brain using 12195-1-AP

    IHC staining of human brain using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human lung using 12195-1-AP

    IHC staining of human lung using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 12195-1-AP (VPS33B antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human placenta using 12195-1-AP

    IHC staining of human placenta using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human testis using 12195-1-AP

    IHC staining of human testis using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human spleen using 12195-1-AP

    IHC staining of human spleen using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human lung using 12195-1-AP

    IHC staining of human lung using 12195-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 12195-1-AP (VPS33B antibody) at dilution of 1:200 (under 40x lens).

  • IF Staining of MCF-7 using 12195-1-AP

    IF Staining of MCF-7 using 12195-1-AP

    Immunofluorescent analysis of MCF-7 cells, using VPS33B antibody 12195-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inHEK-293 cells, mouse skeletal muscle tissue, HeLa cells, mouse testis tissue, L02 cells
Positive IHC detected inhuman colon cancer tissue, human brain tissue, human kidney tissue, human lung tissue, human medulloblastoma tissue, human ovary tissue, human placenta tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12195-1-AP targets VPS33B in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS33B fusion protein Ag2833 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 33 homolog B (yeast)
Calculated molecular weight 617 aa, 71 kDa
Observed molecular weight 65-71 kDa
GenBank accession numberBC016445
Gene symbol VPS33B
Gene ID (NCBI) 26276
RRIDAB_2215198
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS33B, a homolog of yeast class C vacuolar protein sorting (vps) protein Vps33p, belongs to the STXBP/unc-18/SEC1 family. It may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes. VPS33B mediates phagolysosomal fusion in macrophages (PMID: 18474358). Defects in VPS33B account for most cases of arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

Protocols

Product Specific Protocols
WB protocol for VPS33B antibody 12195-1-APDownload protocol
IHC protocol for VPS33B antibody 12195-1-APDownload protocol
IF protocol for VPS33B antibody 12195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF,IP

Nat Genet

Mutations in VIPAR cause an arthrogryposis, renal dysfunction and cholestasis syndrome phenotype with defects in epithelial polarization.

Authors - Cullinane Andrew R AR
  • KD Validated
View Article
mouseWB

Nat Cell Biol

Circadian control of the secretory pathway maintains collagen homeostasis.

Authors - Joan Chang
View Article
humanWB,IF

Proc Natl Acad Sci U S A

Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification.

Authors - Wong Dennis D
View Article
humanIF

J Cell Sci

Late endosomal transport and tethering are coupled processes controlled by RILP and the cholesterol sensor ORP1L.

Authors - van der Kant Rik R
View Article
humanWB

Clin Kidney J

Glomerular involvement in the arthrogryposis, renal dysfunction and cholestasis syndrome.

Authors - Amelia Holme
View Article
humanIHC, WB, CoIP, ChIP

Cancer Sci

VPS33B interacts with NESG1 to suppress cell growth and cisplatin chemoresistance in ovarian cancer.

Authors - Yingxia Ning
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

RICHA (Verified Customer) (11-03-2020)

Excellent antibody for wester and IF.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse fibroblast