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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS25 Monoclonal antibody

VPS25 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rabbit

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3B4C3

Cat no : 68197-1-Ig

Synonyms

DERP9, EAP20, ESCRT II complex subunit VPS25, FAP20, hVps25, VPS25

Validation Data Gallery

Applications:
  • WB analysis using 68197-1-Ig

    WB analysis using 68197-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of rabbit kidney using 68197-1-Ig

    WB analysis of rabbit kidney using 68197-1-Ig

    rabbit kidney tissue were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68197-1-Ig

    WB analysis of HeLa using 68197-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68197-1-Ig (VPS25 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 68197-1-Ig

    IF Staining of HepG2 using 68197-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using VPS25 antibody (68197-1-Ig, Clone: 3B4C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, rabbit kidney tissue, HepG2 cells, Jurkat cells
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy VPS25 Ready-To-Use IHC Kit. VPS25 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

68197-1-Ig targets VPS25 in WB, IF, ELISA applications and shows reactivity with Human, Rabbit samples.

Tested Reactivity Human, Rabbit
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS25 fusion protein Ag8243 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 25 homolog (S. cerevisiae)
Calculated molecular weight 176 aa, 21 kDa
Observed molecular weight19 kDa
GenBank accession numberBC006282
Gene symbol VPS25
Gene ID (NCBI) 84313
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS25 is a component of the endosome-associated complex ESCRT-II (Endosomal Sorting Complexes Required for Transport protein II). ESCRT-II complex, composed of VPS25, VPS36, and SNF8, functions in sorting of ubiquitinated membrane proteins during endocytosis. ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. VPS25 is highly conserved. Human VPS25 gene maps to chromosome 17q21.31 and is expressed in a wide range of tissues (PMID: 16889659).

Protocols

Product Specific Protocols
WB protocol for VPS25 antibody 68197-1-IgDownload protocol
IF protocol for VPS25 antibody 68197-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols