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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VGLUT2 Polyclonal antibody

VGLUT2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29209-1-AP

Synonyms

DNPI, SLC17A6, VGLUT2

Validation Data Gallery

Applications:
  • WB analysis using 29209-1-AP

    WB analysis using 29209-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29209-1-AP (VGLUT2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29209-1-AP

    IHC staining of mouse brain using 29209-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29209-1-AP

    IHC staining of mouse brain using 29209-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29209-1-AP (VGLUT2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 29209-1-AP

    IF Staining of mouse brain using 29209-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse brain using 29209-1-AP

    IF Staining of mouse brain using 29209-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using VGLUT2 antibody (29209-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inunboiled mouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

29209-1-AP targets VGLUT2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VGLUT2 fusion protein Ag29565 相同性解析による交差性が予測される生物種
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 6
Calculated molecular weight 64 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberNM_020346
Gene symbol SLC17A6
Gene ID (NCBI) 57084
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VGLUT2, also known as SLC17A6, belongs to the major facilitator superfamily. VGLUT2 is a multifunctional transporter that transports phosphate at the plasma membrane and glutamate in synaptic vesicles (PMID:33440152, 11432869). VGLUT2 is involved in neurotransmitter loading into synaptic vesicle (PMID: 11698620). VGLUT2 is predominantly expressed in adult and fetal brain, with highest expression in the medulla, substantia nigra, subthalamic nucleus, and thalamus, and low levels in the cerebellum and hippocampus (PMID:10820226).

Protocols

Product Specific Protocols
WB protocol for VGLUT2 antibody 29209-1-APDownload protocol
IHC protocol for VGLUT2 antibody 29209-1-APDownload protocol
IF protocol for VGLUT2 antibody 29209-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols