Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse heart using 14451-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 14451-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 14451-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14451-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14451-1-AP (VDAC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human liver cancer using 14451-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14451-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14451-1-AP (VDAC3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human osteosarcoma using 14451-1-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human osteosarcoma using 14451-1-AP
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using 14451-1-AP (VDAC3 antibody) at dilution of 1:100 (under 10x lens).

VDAC3 Polyclonal antibody

VDAC3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14451-1-AP

Publications(11)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HD VDAC3, hVDAC3, VDAC 3, VDAC3

Formulation: PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (11)

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse heart tissue, human brain tissue, human heart tissue, mouse brain tissue
Positive IHC detected in	human liver cancer tissue, human osteosarcoma tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 10 publications below
IHC	See 2 publications below

Product Information

14451-1-AP targets VDAC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag5960 Product name: Recombinant human VDAC3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-283 aa of BC056870 Sequence: MCNTPTYCDLGKAAKDVFNKGYGFGMVKIDLKTKSCSGVEFSTSGHAYTDTGKASGNLETKYKVCNYGLTFTQKWNTDNTLGTEISWENKLAEGLKLTLDTIFVPNTGKKSGKLKASYKRDCFSVGSNVDIDFSGPTIYGWAVLAFEGWLAGYQMSFDTAKSKLSQNNFALGYKAADFQLHTHVNDGTEFGGSIYQKVNEKIETSINLAWTAGSNNTRFGIAAKYMLDCRTSLSAKVNNASLIGLGYTQTLRPGVKLTLSALIDGKNFSAGGHKVGLGFELEA 相同性解析による交差性が予測される生物種
Full Name	voltage-dependent anion channel 3
Calculated molecular weight	31 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC056870
Gene Symbol	VDAC3
Gene ID (NCBI)	7419
RRID	AB_2212351
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y277
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

Publications

Species	Application	Title
human	WB	Cell Res DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling. Authors - Pengli Zheng View Article
human	WB	Cancer Res miR-450a acts as a tumor suppressor in ovarian cancer by regulating energy metabolism. Authors - Bruna Rodrigues Muys View Article
human	WB	Nat Commun Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models Authors - Ge Gao View Article
mouse	WB	Cell Death Dis TMEM232 is required for the formation of sperm flagellum and male fertility in mice Authors - Xinying Cai View Article
human	WB,IHC	Phytomedicine Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma. Authors - Fuhao Huang View Article
rat	WB	Int J Mol Med Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells. Authors - Lei Sun View Article

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Validation Data Gallery

WB analysis of mouse heart using 14451-1-AP

WB analysis of human brain using 14451-1-AP

WB analysis of human heart using 14451-1-AP

WB analysis of mouse brain using 14451-1-AP

IHC staining of human liver cancer using 14451-1-AP

IHC staining of human liver cancer using 14451-1-AP

IHC staining of human osteosarcoma using 14451-1-AP

IHC staining of human osteosarcoma using 14451-1-AP

VDAC3 Polyclonal antibody

VDAC3 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Publications

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

miR-450a acts as a tumor suppressor in ovarian cancer by regulating energy metabolism.

Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models

TMEM232 is required for the formation of sperm flagellum and male fertility in mice

Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma.

Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells.

Able^™

Validation Data Gallery

WB analysis of mouse heart using 14451-1-AP

WB analysis of human brain using 14451-1-AP

WB analysis of human heart using 14451-1-AP

WB analysis of mouse brain using 14451-1-AP

IHC staining of human liver cancer using 14451-1-AP

IHC staining of human liver cancer using 14451-1-AP

IHC staining of human osteosarcoma using 14451-1-AP

IHC staining of human osteosarcoma using 14451-1-AP

VDAC3 Polyclonal antibody

VDAC3 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Publications

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

miR-450a acts as a tumor suppressor in ovarian cancer by regulating energy metabolism.

Dysregulation of mitochondrial α-ketoglutarate dehydrogenase leads to elevated lipid peroxidation in CHCHD2-linked Parkinson's disease models

TMEM232 is required for the formation of sperm flagellum and male fertility in mice

Hedyotis diffusa injection induces ferroptosis via the Bax/Bcl2/VDAC2/3 axis in lung adenocarcinoma.

Herceptin induces ferroptosis and mitochondrial dysfunction in H9c2 cells.

Able™

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