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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated USP8 Monoclonal antibody

USP8 Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E12E3

Cat no : CL488-67321

Synonyms

Deubiquitinating enzyme 8, FLJ34456, hUBPy, HumORF8, KIAA0055, Ubiquitin isopeptidase Y, ubiquitin specific peptidase 8, Ubiquitin thiolesterase 8, UBPY, USP8

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-67321

    IF Staining of HeLa using CL488-67321

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 USP8 antibody (CL488-67321, Clone: 1E12E3 ) at dilution of 1:200.

  • FC experiment of Hela using CL488-67321

    FC experiment of Hela using CL488-67321

    1X10^6 Hela cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human USP8 (CL488-67321, Clone:1E12E3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (MOPC-21) (CL488-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHela cells
For other applications, we recommend the unconjugated version of this antibody, 67321-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-67321 targets USP8 in IF, FC (Intra) applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP8 fusion protein Ag27104 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 8
Calculated molecular weight 1118 aa, 128 kDa
Observed molecular weight 128 kDa
GenBank accession numberBC110590
Gene symbol USP8
Gene ID (NCBI) 9101
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 USP8 antibody CL488-67321Download protocol
FC protocol for CL Plus 488 USP8 antibody CL488-67321Download protocol
Standard Protocols
Click here to view our Standard Protocols