Validation Data Gallery
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Tested Applications
| Positive WB detected in | MCF-7 cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 11 publications below |
| WB | See 15 publications below |
| IP | See 1 publications below |
| CoIP | See 2 publications below |
| ChIP | See 1 publications below |
Product Information
18066-1-AP targets USP49 in WB, IHC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | USP49 fusion protein Ag12697 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 49 |
| Calculated molecular weight | 73 kDa, 79 kDa |
| Observed molecular weight | 79-85 kDa |
| GenBank accession number | BC014176 |
| Gene Symbol | USP49 |
| Gene ID (NCBI) | 25862 |
| RRID | AB_10643992 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q70CQ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USPs are cysteine proteases that vary greatly in size and structural complexity. Outside of the core catalytic domain, USPs exhibit N-terminal and C-terminal extensions that have been proposed to play roles in determining cellular localization and substrate specificity of these enzymes(PMID:14715245). USP49(Ubiquitin carboxyl-terminal hydrolase 49) belongs to this family. It has 2 isoforms produced by alternative splicing.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for USP49 antibody 18066-1-AP | Download protocol |
| IHC protocol for USP49 antibody 18066-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Differ Genome-wide screening for deubiquitinase subfamily identifies ubiquitin-specific protease 49 as a novel regulator of odontogenesis.
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EMBO J USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.
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Theranostics USP32 confers cancer cell resistance to YM155 via promoting ER-associated degradation of solute carrier protein SLC35F2
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Elife USP49 potently stabilizes APOBEC3G protein by removing ubiquitin and inhibits HIV-1 replication.
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Carcinogenesis USP49 promotes adenocarcinoma of the esophagogastric junction malignant progression via activating SHCBP1-β-catenin-GPX4 axis
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J Cell Mol Med CCAT1/FABP5 promotes tumour progression through mediating fatty acid metabolism and stabilizing PI3K/AKT/mTOR signalling in lung adenocarcinoma. |