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  • KD/KO Validated

USP33 Polyclonal antibody

USP33 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20445-1-AP

Synonyms

Deubiquitinating enzyme 33, hVDU1, KIAA1097, Ubiquitin thiolesterase 33, USP33, VDU1



Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

20445-1-AP targets USP33 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP33 fusion protein Ag14268 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 33
Calculated molecular weight 942 aa, 107 kDa
Observed molecular weight 103 kDa, 107 kDa
GenBank accession numberBC016663
Gene symbol USP33
Gene ID (NCBI) 23032
RRIDAB_10694439
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP33(Ubiquitin carboxyl-terminal hydrolase 33) is also named as KIAA1097, VDU1 and belongs to the peptidase C19 family. This protein, originally identified as a von Hippel-Lindau tumor suppressor (VHL) protein-interacting deubiquitinating enzyme, binds to the Robo1 receptor, and that USP33 is required for Slit responsiveness in breast cancer cells(PMID:19706539). The localization of USP33 is broadly confined to the secretory pathway, with all variants localizing to endoplasmic reticulum-associated structures(PMID:21801292). It has 3 isoforms produced by alternative splicing. This antibody is specific to USP33.

Protocols

Product Specific Protocols
WB protocol for USP33 antibody 20445-1-APDownload protocol
IHC protocol for USP33 antibody 20445-1-APDownload protocol
IF protocol for USP33 antibody 20445-1-APDownload protocol
IP protocol for USP33 antibody 20445-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Nat Commun

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

Authors - Xin Li
humanIF, WB

J Cell Biol

Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins.

Authors - Jacob A Culver
mouseWB

Diabetes

Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination.

Authors - Yuguo Niu
human,mouseWB,IP

EMBO Rep

High salt activates p97 to reduce host antiviral immunity by restricting Viperin induction.

Authors - Yukang Yuan
  • KD Validated
humanWB

Cell Death Dis

NudC-like protein 2 restrains centriole amplification by stabilizing HERC2.

Authors - Min Li
  • KD Validated
humanWB

Oncotarget

NEURL4 regulates the transcriptional activity of tumor suppressor protein p53 by modulating its oligomerization.

Authors - Monica Cubillos-Rojas
  • KD Validated