Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, mouse heart tissue, MDA-MB-231 cells |
| Positive IP detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 13 publications below |
| WB | See 29 publications below |
| IHC | See 7 publications below |
| IF | See 9 publications below |
| IP | See 5 publications below |
| CoIP | See 5 publications below |
Product Information
17707-1-AP targets USP28 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12003 Product name: Recombinant human USP28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC065928 Sequence: MTAELQQDDAAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDERVKEPSQDTVATEPSEVEGSAANKEVLAKVIDLTHDNKDDLQAAIALSLLESPKIQADGRDLNRMHEATSAETKRSKRKRCEVWGENPNPNDWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYSLPQNVLENCRSHTEKRNIMFMQELQYLFALMMGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSPRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYRNLDECLEGAMVEGDVELLPSDHSVKY 相同性解析による交差性が予測される生物種 |
| Full Name | ubiquitin specific peptidase 28 |
| Calculated molecular weight | 583aa,66 kDa; 1077aa,120 kDa |
| Observed molecular weight | 135 kDa |
| GenBank accession number | BC065928 |
| Gene Symbol | USP28 |
| Gene ID (NCBI) | 57646 |
| RRID | AB_2272676 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RU2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
USP28(Ubiquitin carboxyl-terminal hydrolase 28) is also named as KIAA1515. USP28 is a 53BP1-interacting protein and a putative deubiquitinating enzyme, and belongs to a family of enzymes, called deubiquitinating enzymes (DUBs), which remove ubiquitin from the ubiquitin conjugates(PMID:16901786). It can be phosphorylated upon DNA damage at Ser-67 and Ser-714, by ATM or ATR(PMID:16901786). This protein has some isoforms produced by alternative splicing with the molecular mass of 122 kDa, 119 kDa and 66 kDa, and can be detected as 130-140 kDa (PMID: 16901786).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for USP28 antibody 17707-1-AP | Download protocol |
| WB protocol for USP28 antibody 17707-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cancer The deubiquitinase USP25 supports colonic inflammation and bacterial infection and promotes colorectal cancer. | ||
Nat Commun USP5 stabilizes YTHDF1 to control cancer immune surveillance through mTORC1-mediated phosphorylation | ||
Mol Cell Distinct USP25 and USP28 Oligomerization States Regulate Deubiquitinating Activity. | ||
Nucleic Acids Res The dimeric deubiquitinase USP28 integrates 53BP1 and MYC functions to limit DNA damage | ||
J Clin Invest The deubiquitinase USP28 controls intestinal homeostasis and promotes colorectal cancer.
| ||
Adv Sci (Weinh) PFKFB4 Deubiquitination by USP10 Enhances Fumarate Metabolism to Orchestrate the KDM1A/Rad51 Axis and Confer Radioresistance in Lung Cancer. |