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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 555-conjugated USP14 Polyclonal antibody

USP14 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 555 Fluorescent Dye

Cat no : CL555-14517

Synonyms

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL555-14517

    FC experiment of HepG2 using CL555-14517

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 555 Anti-Human USP14 (CL555-14517) (red), or 0.2 ug CoraLite® Plus 555 Rabbit IgG Control Antibody(CL555-30000)(Blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 14517-1-AP

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL555-14517 targets USP14 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP14 fusion protein Ag5979 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Calculated molecular weight 54 kDa
Observed molecular weight 52-60 kDa
GenBank accession numberBC003556
Gene symbol USP14
Gene ID (NCBI) 9097
Conjugate CoraLite® Plus 555 Fluorescent Dye
Excitation/Emission maxima wavelengths554 nm / 570 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.This protein has a 45-kDa catalytic domain (PMID:16211010).

Protocols

Product Specific Protocols
FC protocol for CL Plus 555 USP14 antibody CL555-14517Download protocol
Standard Protocols
Click here to view our Standard Protocols