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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

USP14 Monoclonal antibody

USP14 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig, Rabbit and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3B12G3

Cat no : 67746-1-Ig

Synonyms

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

Validation Data Gallery

Applications:
  • WB analysis using 67746-1-Ig

    WB analysis using 67746-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 67746-1-Ig

    WB analysis using 67746-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67746-1-Ig (USP14 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of HepG2 using 67746-1-Ig

    IF Staining of HepG2 using 67746-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using USP14 antibody (67746-1-Ig, Clone: 3B12G3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHCT 116 cells, HeLa cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, pig brain tissue, rabbit brain tissue, rat brain tissue, mouse brain tissue, U2OS cells, K-562 cells
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

67746-1-Ig targets USP14 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig, Rabbit samples.

Tested Reactivity Human, Mouse, Rat, Pig, Rabbit
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen USP14 fusion protein Ag6312 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Calculated molecular weight 54 kDa
Observed molecular weight60 kDa
GenBank accession numberBC003556
Gene symbol USP14
Gene ID (NCBI) 9097
RRIDAB_2918515
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

Protocols

Product Specific Protocols
WB protocol for USP14 antibody 67746-1-IgDownload protocol
IF protocol for USP14 antibody 67746-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Br J Cancer

GSTM3 enhances radiosensitivity of nasopharyngeal carcinoma by promoting radiation-induced ferroptosis through USP14/FASN axis and GPX4

Authors - Yuting Chen
View Article