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  • Featured Product
  • KD/KO Validated

USP13 Polyclonal antibody

USP13 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16840-1-AP

Synonyms

Deubiquitinating enzyme 13, Isopeptidase T 3, IsoT 3, ISOT3, Ubiquitin thiolesterase 13, USP13

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 16840-1-AP

    WB analysis of HeLa using 16840-1-AP

    WB result of USP13 antibody (16840-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-USP13 transfected HeLa cells.

  • WB analysis using 16840-1-AP

    WB analysis using 16840-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 16840-1-AP

    WB analysis of mouse testis using 16840-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16840-1-AP

    WB analysis of HeLa using 16840-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of A375 using 16840-1-AP

    WB analysis of A375 using 16840-1-AP

    A375 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16840-1-AP

    WB analysis of HEK-293 using 16840-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 16840-1-AP

    WB analysis of HeLa using 16840-1-AP

    WB result of USP13 antibody (16840-1-AP, 1:1000) with si-Control and si-USP13 transfected HeLa cells.

  • WB analysis of rat testis using 16840-1-AP

    WB analysis of rat testis using 16840-1-AP

    rat testis tissue were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 16840-1-AP

    WB analysis of HEK-293 using 16840-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis of PC-3 using 16840-1-AP

    WB analysis of PC-3 using 16840-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 16840-1-AP (USP13 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16840-1-AP

    IP experiment of HeLa using 16840-1-AP

    IP result of anti-USP13 (IP:16840-1-AP, 5ug; Detection:16840-1-AP 1:500) with HeLa cells lysate 2000ug.

  • IHC staining of human testis using 16840-1-AP

    IHC staining of human testis using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 16840-1-AP

    IHC staining of human testis using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human brain using 16840-1-AP

    IHC staining of human brain using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 16840-1-AP

    IHC staining of human brain using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 16840-1-AP

    IHC staining of human heart using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 16840-1-AP

    IHC staining of human heart using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human skin cancer using 16840-1-AP

    IHC staining of human skin cancer using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human skin cancer using 16840-1-AP

    IHC staining of human skin cancer using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 16840-1-AP (USP13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 16840-1-AP

    IHC staining of human liver using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver using 16840-1-AP

    IHC staining of human liver using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human ovary using 16840-1-AP

    IHC staining of human ovary using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 16840-1-AP

    IHC staining of human placenta using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human placenta using 16840-1-AP

    IHC staining of human placenta using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human ovary using 16840-1-AP

    IHC staining of human ovary using 16840-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16840-1-AP (USP13 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 16840-1-AP

    IF Staining of HeLa using 16840-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 16840-1-AP (USP13 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, A375 cells, HEK-293 cells, rat testis tissue, PC-3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human skin cancer tissue, human heart tissue, human placenta tissue, human brain tissue, human liver tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16840-1-AP targets USP13 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen USP13 fusion protein Ag10527 相同性解析による交差性が予測される生物種
Full Name ubiquitin specific peptidase 13 (isopeptidase T-3)
Calculated molecular weight 863 aa, 97 kDa
Observed molecular weight 97-100 kDa
GenBank accession numberBC016146
Gene symbol USP13
Gene ID (NCBI) 8975
RRIDAB_2214569
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for USP13 antibody 16840-1-APDownload protocol
IHC protocol for USP13 antibody 16840-1-APDownload protocol
IF protocol for USP13 antibody 16840-1-APDownload protocol
IP protocol for USP13 antibody 16840-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Genes Dev

Chaperone-mediated autophagy degrades mutant p53.

Authors - Vakifahmetoglu-Norberg Helin H
View Article
human,mouseWB,IHC,IF

EBioMedicine

The deubiquitinase USP13 stabilizes the anti-inflammatory receptor IL-1R8/Sigirr to suppress lung inflammation.

Authors - Lian Li
  • KD Validated
View Article
mouse,humanWB,IF

Transl Res

Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.

Authors - Xinxin Liao
  • KO Validated
View Article
humanWB

J Cell Biol

Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death.

Authors - Hong-Guang Xia
View Article
humanWB,IP

Oncogene

USP13 deubiquitinates and stabilizes cyclin D1 to promote gastric cancer cell cycle progression and cell proliferation

Authors - Cunying Ma
  • KD Validated
View Article
mouseIP

J Cell Physiol

IL-37-induced activation of glycogen synthase kinase 3β promotes IL-1R8/Sigirr phosphorylation, internalization, and degradation in lung epithelial cells.

Authors - Lian Li
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Francesca (Verified Customer) (05-22-2019)

Very good antibody for western blot. Antibody specificity validated by USP13 knock-down.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1: 2000 in 5% milk
  • Cell Tissue Type: MeT-5A cells
USP13 Antibody Western Blot, validation (1: 2000 in 5% milk dilution) in MeT-5A cells (Cat no:16840-1-AP)