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  • KD/KO Validated

UQCRQ Polyclonal antibody

UQCRQ Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14975-1-AP

Synonyms

Complex III subunit 8, Complex III subunit VIII, QCR8, QP C, QPC, UQCRQ



Tested Applications

Positive WB detected inHepG2 cells, human liver tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy UQCRQ Ready-To-Use IHC Kit. UQCRQ primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14975-1-AP targets UQCRQ in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UQCRQ fusion protein Ag6886 相同性解析による交差性が予測される生物種
Full Name ubiquinol-cytochrome c reductase, complex III subunit VII, 9.5kDa
Calculated molecular weight 10 kDa
Observed molecular weight 10 kDa
GenBank accession numberBC001390
Gene symbol UQCRQ
Gene ID (NCBI) 27089
RRIDAB_2288356
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UQCRQ(Cytochrome b-c1 complex subunit 8) belongs to the UQCRQ/QCR8 family. UQCRQ is a ubiquinone-binding protein localized to the cytochrome bc1 region of the mitochondrial respiratory chain. The deduced 110-amino acid protein has a relatively hydrophobic and basic N-terminal region, an aspartic acid-rich middle region, and a glutamic acid- and lysine-rich C-terminal region.

Protocols

Product Specific Protocols
WB protocol for UQCRQ antibody 14975-1-APDownload protocol
IHC protocol for UQCRQ antibody 14975-1-APDownload protocol
IF protocol for UQCRQ antibody 14975-1-APDownload protocol
IP protocol for UQCRQ antibody 14975-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Deficiency of T-Cell Intracellular Antigen 1 in Murine Embryonic Fibroblasts Is Associated with Changes in Mitochondrial Morphology and Respiration.

Authors - Isabel Carrascoso
humanWB

Stem Cells

The Transcription Factor 7-Like 2-Peroxisome Proliferator-Activated Receptor Gamma Coactivator-1 Alpha Axis Connects Mitochondrial Biogenesis and Metabolic Shift with Stem Cell Commitment to Hepatic Differentiation.

Authors - Anaïs Wanet
ratWB

Cell Death Dis

Calcium sensing receptor protects high glucose-induced energy metabolism disorder via blocking gp78-ubiquitin proteasome pathway.

Authors - Yuehong Wang
human

J Biol Chem

Mitochondrial reactive oxygen species regulate transforming growth factor-β signaling.

Authors - Jain Manu M
  • KD Validated
mouseWB

Am J Physiol Endocrinol Metab

Exogenous H2S reduces acetylation levels of mitochondrial respiratory enzymes via regulating NAD+-SIRT3 pathway in cardiac tissues of db/db mice.

Authors - Yu Sun
mouseWB

Front Endocrinol (Lausanne)

The Impaired Bioenergetics of Diabetic Cardiac Microvascular Endothelial Cells.

Authors - Haitao Zhang