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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

UPF3A Polyclonal antibody

UPF3A Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17114-1-AP

Synonyms

hUpf3, HUPF3A, RENT3A, UPF3, UPF3A

Validation Data Gallery

Applications:
  • WB analysis using 17114-1-AP

    WB analysis using 17114-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 17114-1-AP

    WB analysis of HeLa using 17114-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 17114-1-AP

    WB analysis of human testis using 17114-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 17114-1-AP (UPF3A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 17114-1-AP

    IHC staining of human testis using 17114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human testis using 17114-1-AP

    IHC staining of human testis using 17114-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 17114-1-AP (UPF3A Antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human heart using 17114-1-AP

    IHC staining of human heart using 17114-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 17114-1-AP

    IHC staining of human heart using 17114-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 17114-1-AP (UPF3A antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 17114-1-AP

    IF Staining of HeLa using 17114-1-AP

    Immunofluorescent analysis of HeLa cells using 17114-1-AP (UPF3A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, human testis tissue, Jurkat cells
Positive IHC detected inhuman testis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17114-1-AP targets UPF3A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UPF3A fusion protein Ag10799 相同性解析による交差性が予測される生物種
Full Name UPF3 regulator of nonsense transcripts homolog A (yeast)
Calculated molecular weight 443 aa, 51 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC023569
Gene symbol UPF3A
Gene ID (NCBI) 65110
RRIDAB_10694141
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UPF3A antibody 17114-1-APDownload protocol
IHC protocol for UPF3A antibody 17114-1-APDownload protocol
IF protocol for UPF3A antibody 17114-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Manag Res

Functions and Clinical Significance of UPF3a Expression in Human Colorectal Cancer.

Authors - Xinmin Bao
  • KD Validated
View Article
humanWB

Biochim Biophys Acta

Increase of a Group of PTC(+) Transcripts by Curcumin through Inhibition of the NMD Pathway.

Authors - Dairong Feng
View Article
humanIHC

J Gastroenterol Hepatol

Genetic compensation response could exist in colorectal cancer: UPF3A up-regulates the oncogenic homologue gene SRSF3 expression corresponding to SRSF6 to promote colorectal cancer metastasis

Authors - Weimin Xu
View Article