Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervix tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 1 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| ELISA | See 1 publications below |
Product Information
15479-1-AP targets UNC45A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, fish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7790 Product name: Recombinant human UNC45A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 722-944 aa of BC006214 Sequence: EMTFPGERIYEVVRPLVSLLHLNCSGLQNFEALMALTNLAGISERLRQKILKEKAVPMIEGYMFEEHEMIRRAATECMCNLAMSKEVQDLFEAQGNDRLKLLVLYSGEDDELLQRAAAGGLAMLTSMRPTLCSRIPQVTTHWLEILQALLLSSNQELQHRGAVVVLNMVEASREIASTLMESEMMEILSVLAKGDHSPVTRAAAACLDKAVEYGLIQPNQDGE 相同性解析による交差性が予測される生物種 |
| Full Name | unc-45 homolog A (C. elegans) |
| Calculated molecular weight | 103 kDa |
| Observed molecular weight | 100 kDa |
| GenBank accession number | BC006214 |
| Gene Symbol | UNC45A |
| Gene ID (NCBI) | 55898 |
| RRID | AB_2212736 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H3U1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UNC45A antibody 15479-1-AP | Download protocol |
| IHC protocol for UNC45A antibody 15479-1-AP | Download protocol |
| IP protocol for UNC45A antibody 15479-1-AP | Download protocol |
| WB protocol for UNC45A antibody 15479-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep A Cas9 Ribonucleoprotein Platform for Functional Genetic Studies of HIV-Host Interactions in Primary Human T Cells.
| ||
Food Res Int Inhibitory effect of edible natural compounds with di- and tri-carboxyl moiety on endogenous protease inducing disassembly and degradation of myofibrils from grass carp (Ctenopharyngodon idella). | ||
Cancer Biol Ther UNC-45A is preferentially expressed in epithelial cells and binds to and co-localizes with interphase MTs. | ||
J Biol Chem Quantitative interactome proteomics identifies a proteostasis network for GABAA receptors | ||
Gastroenterology Myosin Vb Traffics P-glycoprotein to the Apical Membrane of Intestinal Epithelial Cells |
