Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse colon tissue, COLO 320 cells, HeLa cells, Raji cells | 
| Positive IP detected in | COLO 320 cells | 
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 | 
| Positive IF/ICC detected in | Hela cells, MCF-7 cells | 
Recommended dilution
| Application | Dilution | 
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 | 
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate | 
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 | 
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 | 
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13642-1-AP targets UIMC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse | 
| Host / Isotype | Rabbit / IgG | 
| Class | Polyclonal | 
| Type | Antibody | 
| Immunogen | CatNo: Ag4582 Product name: Recombinant human UIMC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-553 aa of BC032561 Sequence: DQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQSSQGIVEETSEEGNSVPASQSVAALTSKRSLVLMPESSAEEITVCPETQLSSSETFDLEREVSPGSRDILDGVRIIMADKEVGNKEDAEKEVAISTFSSSNQVSCPLCDQCFPPTKIERHAMYCNGLMEEDTVLTRRQKEAKTKSDSGTAAQTSLDIDKNEKCYLCKSLVPFREYQCHVDSCLQLAKADQGDGPEGSGRACSTVEGKWQQRLKNPKEKGHSEGRLLSFLEQSEHKTSDADIKSSETGAFRVPSPGMEEAGCSREMQSSFTRRDLNESPVKSFVSISEATDCLVDFKKQVTVQPGSRTRTKAGRGRRRKF相同性解析による交差性が予測される生物種 | 
| Full Name | ubiquitin interaction motif containing 1 | 
| Calculated molecular weight | 719 aa, 80 kDa | 
| Observed molecular weight | 97 kDa, 80 kDa, 71-75 kDa, 61 kDa | 
| GenBank accession number | BC032561 | 
| Gene Symbol | UIMC1 | 
| Gene ID (NCBI) | 51720 | 
| RRID | AB_10641052 | 
| Conjugate | Unconjugated | 
| Form | |
| Form | Liquid | 
| Purification Method | Antigen affinity purification | 
| UNIPROT ID | Q96RL1 | 
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 | 
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. | 
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UIMC1 antibody 13642-1-AP | Download protocol | 
| IHC protocol for UIMC1 antibody 13642-1-AP | Download protocol | 
| IP protocol for UIMC1 antibody 13642-1-AP | Download protocol | 
| WB protocol for UIMC1 antibody 13642-1-AP | Download protocol | 
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols | 












