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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using UHRF1 Polyclonal antibody (21402-1-AP)

    WB analysis using 21402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using UHRF1 Polyclonal antibody (21402-1-AP)

    WB analysis of HEK-293T using 21402-1-AP

    WB result of UHRF1 antibody (21402-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-UHRF1 transfected HEK-293T cells.

  • Western Blot (WB) analysis of HeLa cells using UHRF1 Polyclonal antibody (21402-1-AP)

    WB analysis of HeLa using 21402-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using UHRF1 Polyclonal antibody (21402-1-AP)

    IP experiment of HeLa using 21402-1-AP

    IP result of anti-UHRF1 (IP:21402-1-AP, 4ug; Detection:21402-1-AP 1:1000) with HeLa cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of rat thymus tissue using UHRF1 Polyclonal antibody (21402-1-AP)

    IHC staining of rat thymus using 21402-1-AP

    Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 21402-1-AP (UHRF1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse thymus tissue using UHRF1 Polyclonal antibody (21402-1-AP)

    IHC staining of mouse thymus using 21402-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 21402-1-AP (UHRF1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using UHRF1 Polyclonal antibody (21402-1-AP)

    IHC staining of human lung cancer using 21402-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21402-1-AP (UHRF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using UHRF1 Polyclonal antibody (21402-1-AP)

    IF Staining of HepG2 using 21402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using UHRF1 antibody (21402-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO Validated

UHRF1 Polyclonal antibody

UHRF1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21402-1-AP
Publications(35)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

E3 ubiquitin-protein ligase UHRF1, EC:2.3.2.27, hNp95, hUHRF1, HuNp95

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHuH-7 cells, HEK-293T cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, mouse thymus tissue, rat thymus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21402-1-AP targets UHRF1 in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16111

Product name: Recombinant human UHRF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 88-392 aa of BC113875

Sequence: QSLVLPHSTKERDSELSDTDSGCCLGQSESDKSSTHGEAAAETDSRPADEDMWDETELGLYKVNEYVDARDTNMGAWFEAQVVRVTRKAPSRDEPCSSTSRPALEEDVIYHVKYDDYPENGVVQMNSRDVRARARTIIKWQDLEVGQVVMLNYNPDNPKERGFWYDAEISRKRETRTARELYANVVLGDDSLNDCRIIFVDEVFKIERPGEGSPMVDNPMRRKSGPSCKHCKDDVNRLCRVCACHLCGGRQDPDKQLMCDECDMAFHIYCLDPPLSSVPSEDEWYCPECRNDASEVVLAGERLRE

相同性解析による交差性が予測される生物種
Full Name ubiquitin-like with PHD and ring finger domains 1
Calculated molecular weight 806 aa, 91 kDa
Observed molecular weight 91-100 kDa
GenBank accession numberBC113875
Gene Symbol UHRF1
Gene ID (NCBI) 29128
RRIDAB_10860902
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96T88
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UHRF1, also named as ICBP90, NP95 and RNF106, is a putative E3 ubiquitin-protein ligase. It may participate in methylation-dependent transcriptional regulation. UHRF1 binds to inverted 5'-CCAAT-3' box 2 in the TOP2A promoter, and activates TOP2A expression. It is important for G1/S transition and may be involved in DNA repair and chromosomal stability. UHRF1's ability to repress its direct target gene expression is dependent on PHD(UHRF1) binding to unmodified H3R2. In addition, UHRF1 is a novel metabolic guardian restricting AMPK activity(PMID: 34907338).

Protocols

Product Specific Protocols
IF protocol for UHRF1 antibody 21402-1-APDownload protocol
IHC protocol for UHRF1 antibody 21402-1-APDownload protocol
IP protocol for UHRF1 antibody 21402-1-APDownload protocol
WB protocol for UHRF1 antibody 21402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Cancer Cell

Methionine restriction promotes cGAS activation and chromatin untethering through demethylation to enhance antitumor immunity

Authors - Lan Fang
  • KD Validated
View Article
humanIF

Nat Cell Biol

Single-cell multi-omics sequencing of human spermatogenesis reveals a DNA demethylation event associated with male meiotic recombination

Authors - Yaping Huang
View Article
humanWB

Cell Discov

USP7 negatively controls global DNA methylation by attenuating ubiquitinated histone-dependent DNMT1 recruitment.

Authors - Jialun Li
  • KD Validated
View Article
humanWB,IF

Nucleic Acids Res

SET8 prevents excessive DNA methylation by methylation-mediated degradation of UHRF1 and DNMT1.

Authors - Huifang Zhang
View Article
humanWB

Theranostics

A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.

Authors - Qi Zhang
View Article
humanWB

Adv Sci (Weinh)

HDAC2-Mediated METTL3 Delactylation Promotes DNA Damage Repair and Chemotherapy Resistance in Triple-Negative Breast Cancer

Authors - Xiaoniu He
View Article
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