Validation Data Gallery
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Tested Applications
| Positive WB detected in | HEK-293 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
21052-1-AP targets UBXD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13440 Product name: Recombinant human UBXN4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 160-508 aa of BC035594 Sequence: TSDTKSDTATGGESAGHATSSQEPSGCSDQRPAEDLNIRVERLTKKLEERREEKRKEEEQREIKKEIERRKTGKEMLDYKRKQEEELTKRMLEERNREKAEDRAARERIKQQIALDRAERAARFAKTKEEVEAAKAAALLAKQAEMEVKRESYARERSTVARIQFRLPDGSSFTNQFPSDAPLEEARQFAAQTVGNTYGNFSLATMFPRREFTKEDYKKKLLDLELAPSASVVLLPAGRPTASIVHSSSGDIWTLLGTVLYPFLAIWRLISNFLFSNPPPTQTSVRVTSSEPPNPASSSKSEKREPVRKRVLEKRGDDFKKEGKIYRLRTQDDGEDENNTWNGNSTQQM 相同性解析による交差性が予測される生物種 |
| Full Name | UBX domain protein 4 |
| Calculated molecular weight | 508 aa, 57 kDa |
| Observed molecular weight | 64-68 kDa |
| GenBank accession number | BC035594 |
| Gene Symbol | UBXD2 |
| Gene ID (NCBI) | 23190 |
| RRID | AB_10732809 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92575 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| IHC protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| IP protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| WB protocol for UBXD2 antibody 21052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Clin Invest Inadequate ubiquitination-proteasome coupling contributes to myocardial ischemia-reperfusion injury. | ||
Apoptosis Acetylcholine exerts cytoprotection against hypoxia/reoxygenation-induced apoptosis, autophagy and mitochondrial impairment through both muscarinic and nicotinic receptors. | ||
EMBO Rep UBXD8 mediates mitochondria-associated degradation to restrain apoptosis and mitophagy | ||
Nat Commun The p97-UBXD8 complex regulates ER-Mitochondria contact sites by altering membrane lipid saturation and composition | ||
