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Validation Data Gallery

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  • Western Blot (WB) analysis of human brain tissue using UBQLNL Polyclonal antibody (16400-1-AP)

    WB analysis of human brain using 16400-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 16400-1-AP (UBQLNL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using UBQLNL Polyclonal antibody (16400-1-AP)

    WB analysis of Jurkat using 16400-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16400-1-AP (UBQLNL antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Jurkat cells using UBQLNL Polyclonal antibody (16400-1-AP)

    IP experiment of Jurkat using 16400-1-AP

    IP result of anti-UBQLNL (IP:16400-1-AP, 4ug; Detection:16400-1-AP 1:500) with Jurkat cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using UBQLNL Polyclonal antibody (16400-1-AP)

    IHC staining of human colon using 16400-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16400-1-AP (UBQLNL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using UBQLNL Polyclonal antibody (16400-1-AP)

    IHC staining of human colon using 16400-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16400-1-AP (UBQLNL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using UBQLNL Polyclonal antibody (16400-1-AP)

    IF Staining of A431 using 16400-1-AP

    Immunofluorescent analysis of A431 cells using 16400-1-AP (UBQLNL antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using UBQLNL Polyclonal antibody (16400-1-AP)

    IF Staining of A431 using 16400-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using UBQLNL antibody (16400-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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UBQLNL Polyclonal antibody

UBQLNL Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16400-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Ubiquilin-like protein, Ubiquilin like protein, ubiquilin like

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman brain tissue, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16400-1-AP targets UBQLNL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBQLNL fusion protein Ag9496 相同性解析による交差性が予測される生物種
Full Name ubiquilin-like
Calculated molecular weight 475 aa, 53 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC012183
Gene Symbol UBQLNL
Gene ID (NCBI) 143630
RRIDAB_2878253
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IYU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBQLNL also named as Ubiquilin like protein, encodes a 475 amino acid protein. The specific function of it is still non-known. UBQLNL may have an association with type 2 diabetes, but the mechanism is supposed be studied in the further research. The subcellular localization of UBQLNL is nucleus. The expression of it is detected in some tissues, such as brain ,testis, heart and liver.

Protocols

Product Specific Protocols
WB protocol for UBQLNL antibody 16400-1-APDownload protocol
IHC protocol for UBQLNL antibody 16400-1-APDownload protocol
IF protocol for UBQLNL antibody 16400-1-APDownload protocol
IP protocol for UBQLNL antibody 16400-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Mol Med Rep

Decreased expression of ubiquilin‑1 following neonatal hypoxia‑ischemic brain injury in mice.

Authors - Li Luo
View Article