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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

UBLCP1 Polyclonal antibody

UBLCP1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12099-1-AP

Synonyms

CPUB1, UBLCP1

Validation Data Gallery

Applications:
  • WB analysis of K-562 using 12099-1-AP

    WB analysis of K-562 using 12099-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12099-1-AP (UBLCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 12099-1-AP

    WB analysis of human brain using 12099-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 12099-1-AP (UBLCP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of K-562 using 12099-1-AP

    IP experiment of K-562 using 12099-1-AP

    IP result of anti-UBLCP1 (IP:12099-1-AP, 3ug; Detection:12099-1-AP 1:500) with K-562 cells lysate 1760ug.

  • IHC staining of human ovary tumor using 12099-1-AP

    IHC staining of human ovary tumor using 12099-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12099-1-AP (UBLCP1 antibody) at dilution of 1:50 (under 10x lens).

  • IF Staining of HeLa using 12099-1-AP

    IF Staining of HeLa using 12099-1-AP

    Immunofluorescent analysis of Hela cells, using UBLCP1 antibody 12099-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inK-562 cells, human brain tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12099-1-AP targets UBLCP1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBLCP1 fusion protein Ag2743 相同性解析による交差性が予測される生物種
Full Name ubiquitin-like domain containing CTD phosphatase 1
Calculated molecular weight 318 aa, 37 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC013425
Gene symbol UBLCP1
Gene ID (NCBI) 134510
RRIDAB_2256901
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for UBLCP1 antibody 12099-1-APDownload protocol
IHC protocol for UBLCP1 antibody 12099-1-APDownload protocol
IF protocol for UBLCP1 antibody 12099-1-APDownload protocol
IP protocol for UBLCP1 antibody 12099-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols