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  • KD/KO Validated

UBE2T/HSPC150 Polyclonal antibody

UBE2T/HSPC150 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10105-2-AP

Synonyms

HSPC150, PIG50, UBE2T, UBE2T/HSPC150, Ubiquitin carrier protein T, Ubiquitin protein ligase T



Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, Jurkat cells, SKOV-3 cells
Positive IP detected inHeLa cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

10105-2-AP targets UBE2T/HSPC150 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBE2T/HSPC150 fusion protein Ag0153 相同性解析による交差性が予測される生物種
Full Name ubiquitin-conjugating enzyme E2T (putative)
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC004152
Gene symbol UBE2T
Gene ID (NCBI) 29089
RRIDAB_2211478
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The ubiquitin (Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic (UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs (TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.

Protocols

Product Specific Protocols
WB protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IF protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
IP protocol for UBE2T/HSPC150 antibody 10105-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Am J Hum Genet

Mutations in the Gene Encoding the E2 Conjugating Enzyme UBE2T Cause Fanconi Anemia.

Authors - Asuka Hira
humanWB

Cancer Res

Proteasome function is required for DNA damage response and fanconi anemia pathway activation.

Authors - Céline Jacquemont
humanWB

Blood Adv

Amplification and overexpression of E2 ubiquitin conjugase UBE2T promotes homologous recombination in multiple myeloma.

Authors - David A Alagpulinsa
humanWB,IHC

Oncotarget

UBE2T promotes nasopharyngeal carcinoma cell proliferation, invasion, and metastasis by activating the AKT/GSK3β/β-catenin pathway.

Authors - Wei Hu
  • KD Validated
humanWB

Biomed Pharmacother

Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.

Authors - Shuai Huang

Tumour Biol

Ubiquitin-Conjugating Enzyme E2T is an Independent Prognostic Factor and Promotes Gastric Cancer Progression.

Authors - Hao Yu