• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

  • Featured Product
  • KD/KO Validated

UBAP1 Polyclonal antibody

UBAP1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 12385-1-AP

Synonyms

NAG20, UAP, UBAP, UBAP 1, UBAP1, ubiquitin associated protein 1

Validation Data Gallery

Applications:
  • WB analysis of human heart using 12385-1-AP

    WB analysis of human heart using 12385-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 12385-1-AP

    WB analysis of Y79 using 12385-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human lung using 12385-1-AP

    WB analysis of human lung using 12385-1-AP

    human lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse lung using 12385-1-AP

    WB analysis of mouse lung using 12385-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 12385-1-AP

    WB analysis of mouse brain using 12385-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 12385-1-AP

    WB analysis of A431 using 12385-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 12385-1-AP

    WB analysis of human testis using 12385-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human spleen using 12385-1-AP

    WB analysis of human spleen using 12385-1-AP

    human spleen tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 12385-1-AP

    WB analysis of mouse testis using 12385-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse thymus using 12385-1-AP

    WB analysis of mouse thymus using 12385-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 12385-1-AP

    WB analysis of human testis using 12385-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human testis using 12385-1-AP

    WB analysis of human testis using 12385-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12385-1-AP (UBAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse brain using 12385-1-AP

    IP experiment of mouse brain using 12385-1-AP

    IP result of anti-UBAP1 (IP:12385-1-AP, 4ug; Detection:12385-1-AP 1:500) with mouse brain tissue lysate 4000ug.

  • IHC staining of human lung cancer using 12385-1-AP

    IHC staining of human lung cancer using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 12385-1-AP

    IHC staining of human lung cancer using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12385-1-AP (UBAP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 12385-1-AP

    IHC staining of human lung cancer using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung cancer using 12385-1-AP

    IHC staining of human lung cancer using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung using 12385-1-AP

    IHC staining of human lung using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human lung using 12385-1-AP

    IHC staining of human lung using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human lung using 12385-1-AP

    IHC staining of human lung using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human lung using 12385-1-AP

    IHC staining of human lung using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 12385-1-AP (UBAP1 antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human heart using 12385-1-AP

    IHC staining of human heart using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 12385-1-AP

    IHC staining of human heart using 12385-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 12385-1-AP (UBAP1 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 12385-1-AP

    IF Staining of HeLa using 12385-1-AP

    Immunofluorescent analysis of Hela cells, using UBAP1 antibody 12385-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inhuman heart tissue, A431 cells, human lung tissue, human spleen tissue, human testis tissue, mouse brain tissue, mouse lung tissue, mouse testis tissue, mouse thymus tissue, Y79 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman lung cancer tissue, human heart tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

12385-1-AP targets UBAP1 in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen UBAP1 fusion protein Ag3055 相同性解析による交差性が予測される生物種
Full Name ubiquitin associated protein 1
Calculated molecular weight 502 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC020950
Gene symbol UBAP1
Gene ID (NCBI) 51271
RRIDAB_2211886
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

UBAP1, also named as NAG20, is a 55 kDa protein which contains 2 putative tandem UBA domains at the C terminus, a coiled-coil domain, 2 possible N-glycosylation sites, 5 PKC phosphorylation sites, 7 casein kinase II phosphorylation sites, and 3 N-myristoylation sites. UBAP1 might be a potential effective diagnosis candidate for NPC and decreased expression of UBAP1 protein is a possible point of dysfunction along the pathogenesis pathway for NPC that may contribute to malignant transformation.

Protocols

Product Specific Protocols
WB protocol for UBAP1 antibody 12385-1-APDownload protocol
IHC protocol for UBAP1 antibody 12385-1-APDownload protocol
IF protocol for UBAP1 antibody 12385-1-APDownload protocol
IP protocol for UBAP1 antibody 12385-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO J

A Pseudomonas aeruginosa TIR effector mediates immune evasion by targeting UBAP1 and TLR adaptors.

Authors - Paul Rc Imbert
View Article
humanWB

Curr Biol

UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting.

Authors - Stefani Flavia F
View Article
humanWB

J Cell Biol

VPS37A directs ESCRT recruitment for phagophore closure.

Authors - Yoshinori Takahashi
View Article
humanWB

Cell Rep

The Dynamics of TGF-β Signaling Are Dictated by Receptor Trafficking via the ESCRT Machinery.

Authors - Daniel S J Miller
View Article
humanWB, IP

Cell Rep

Interplay of Endosomal pH and Ligand Occupancy in Integrin α5β1 Ubiquitination, Endocytic Sorting, and Cell Migration.

Authors - Dmitri Kharitidi
View Article
humanWB,IP

PLoS Pathog

A cytoplasmic tail determinant in HIV-1 Vpu mediates targeting of tetherin for endosomal degradation and counteracts interferon-induced restriction.

Authors - Kueck Tonya T
View Article