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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Talin-1 Polyclonal antibody

Talin-1 Polyclonal Antibody for IF, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-14168

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL488-14168

    IF Staining of A549 using CL488-14168

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 Talin-1 antibody (CL488-14168) at dilution of 1:200.

  • FC experiment of HeLa using CL488-14168

    FC experiment of HeLa using CL488-14168

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Talin-1 (CL488-14168) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA549 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14168-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-14168 targets Talin-1 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Talin-1 fusion protein Ag5367 相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight 270 kDa, 230 kDa
GenBank accession numberBC042923
Gene symbol TLN1
Gene ID (NCBI) 7094
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Talin-1 antibody CL488-14168Download protocol
Standard Protocols
Click here to view our Standard Protocols