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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TYK2 Monoclonal antibody

TYK2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1E1A10

Cat no : 67411-1-Ig

Synonyms

JTK1, TYK2, tyrosine kinase 2

Validation Data Gallery

Applications:
  • WB analysis using 67411-1-Ig

    WB analysis using 67411-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • WB analysis using 67411-1-Ig

    WB analysis using 67411-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67411-1-Ig (TYK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • IHC staining of human colon cancer using 67411-1-Ig

    IHC staining of human colon cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67411-1-Ig

    IHC staining of human colon cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67411-1-Ig

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67411-1-Ig

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67411-1-Ig

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 67411-1-Ig

    IHC staining of human liver cancer using 67411-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67411-1-Ig (TYK2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 67411-1-Ig

    IF Staining of HepG2 using 67411-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using TYK2 antibody (67411-1-Ig, Clone: 1E1A10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, HEK-293 cells, A549 cells, Jurkat cells, THP-1 cells, HSC-T6 cells
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 4 publications below

Product Information

67411-1-Ig targets TYK2 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TYK2 fusion protein Ag9442 相同性解析による交差性が予測される生物種
Full Name tyrosine kinase 2
Calculated molecular weight 1187 aa, 134 kDa
Observed molecular weight 134 kDa
GenBank accession numberBC014243
Gene symbol TYK2
Gene ID (NCBI) 7297
RRIDAB_2882652
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TYK2 antibody 67411-1-IgDownload protocol
IHC protocol for TYK2 antibody 67411-1-IgDownload protocol
IF protocol for TYK2 antibody 67411-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Virol

SARS-CoV-2 disrupts proximal elements in the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article
humanWB

Invest New Drugs

IL-10 contributes to gemcitabine resistance in extranodal NK/T-cell lymphoma cells via ABCC4.

Authors - Jia Huo
View Article
humanWB

bioRxiv

SARS-CoV-2 desensitizes host cells to interferon through inhibition of the JAK-STAT pathway.

Authors - Da-Yuan Chen
View Article
humanWB

Cancer Lett

HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma

Authors - Xueshuo Sun
View Article