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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TXNIP Polyclonal antibody

TXNIP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27429-1-AP

Synonyms

EST01027, HHCPA78, THIF, Thioredoxin binding protein 2, TXNIP, VDUP1

Validation Data Gallery

Applications:
  • WB analysis using 27429-1-AP

    WB analysis using 27429-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27429-1-AP (TXNIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 27429-1-AP

    IHC staining of mouse colon using 27429-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 27429-1-AP

    IHC staining of mouse colon using 27429-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 27429-1-AP (TXNIP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 27429-1-AP

    IF Staining of HeLa using 27429-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using TXNIP antibody (27429-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, MCF-7 cells, RAW 264.7 cells
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

27429-1-AP targets TXNIP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TXNIP fusion protein Ag26579 相同性解析による交差性が予測される生物種
Full Name thioredoxin interacting protein
Calculated molecular weight 391 aa, 44 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC093702
Gene symbol TXNIP
Gene ID (NCBI) 10628
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TXNIP, also known as VDUP-1 or TBP-2, belongs to alpha-arrestin protein family and is perhaps the only family member known to bind thioredoxin (TRX). TXNIP was induced by Vitamin D3, but not induced by another monocyte or macrophage differentiation inducer: phorbol 12-myristate 13-acetate (PMA). TXNIP bound catalytic active center of thioredoxin (TRX), which protected cells against oxidative stress. TXNIP was found to be a negative regulator of thioredoxin activity and inducer of the intracellular level of reactive oxygen species (ROS). TXNIP plays an important role in a wide variety of biological functions, such as the regulation of cell death, growth, differentiation, and energy metabolism.

Protocols

Product Specific Protocols
WB protocol for TXNIP antibody 27429-1-APDownload protocol
IHC protocol for TXNIP antibody 27429-1-APDownload protocol
IF protocol for TXNIP antibody 27429-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols