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Beta Tubulin 抗体 162 文献

Rabbit Polyclonal| カタログ番号: 10068-1-AP

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Related Primary Antibodies

適用アプリケーション

アプリケーション:
WB, IP, IHC, IF, FC, ELISA
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Images See all

Beta Tubulin Antibody WB HEK-293 cells 10068-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 10068-1-AP (beta Tubulin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Beta Tubulin Antibody IF C2C12 cells 10068-1-AP

Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Beta Tubulin antibody (10068-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Beta Tubulin Antibody IP HEK-293 cells 10068-1-AP

IP result of anti-beta Tubulin (IP:10068-1-AP, 4ug; Detection:10068-1-AP 1:5000) with HEK-293 cells lysate 2320 ug.

Beta Tubulin Antibody IHC mouse brain tissue 10068-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 10x lens).

Beta Tubulin Antibody IHC mouse brain tissue 10068-1-AP

Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10068-1-AP (beta Tubulin antibody at dilution of 1:50 (under 40x lens).

Beta Tubulin Antibody FC HeLa cells 10068-1-AP

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Beta Tubulin (10068-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

種特異性:
human, mouse, rat, bovine, Caenorhabditis elegans, canine, mosue, pig, silkworm, zebrafish
WB ポジティブ:
HEK-293 cells, mouse cerebellum tissue
IP ポジティブ:
HEK-293 cells
IHC ポジティブ:
mouse brain tissue, human brain tissue, rat brain tissue
IF ポジティブ:
C2C12 cells, HepG2 cells
FC ポジティブ:
HeLa cells
推奨希釈倍率:
WB : 1:1000-1:8000
IP : 0.5-4.0 ug for IP and 1:2000-1:10000 for WB
IHC : 1:20-1:200
IF : 1:200-1:800

Protocols

Product-specific protocols

Standard Protocols

製品情報

由来:
Rabbit
精製方法:
Antigen affinity purification
アイソタイプ:
IgG
バッファー/保存方法:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

ターゲット情報

免疫原:
Beta Tubulin fusion protein Ag0117
正式名:
tubulin, beta 3
計算分子量:
450 aa, 50 kDa
検出分子量:
50-55 kDa
GenBank accession number:
BC000748
Gene ID (NCBI):
10381
遺伝子シンボル:
TUBB3
別名:
beta 4, beta tubulin, B-tubulin, MC1R, TUBB, tubulin

背景説明

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha, beta, and gamma tubulins have all been used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.


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