Validation Data Gallery
Tested Applications
| Positive WB detected in | K-562 cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse pancreas tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| FC | See 1 publications below |
Product Information
18695-1-AP targets TSPAN7 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | tetraspanin 7 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 28-35 kDa |
| GenBank accession number | BC018036 |
| Gene Symbol | TSPAN7 |
| Gene ID (NCBI) | 7102 |
| RRID | AB_2213450 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P41732 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TSPAN7, also named as A15, MXS1, CD231, MRX58, CCG-B7, TM4SF2, TALLA-1, TM4SF2b and DXS1692E, is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. TSPAN7 is a cell surface glycoprotein and may have a role in the control of neurite outgrowth. TSPAN7 may be involved in cell proliferation and cell motility. It is not solely expressed in T cells, but also expressed in acute myelocytic leukemia cells of some patients. The antibody only recognize the protein TSPAN7, can't recognize the protein TSPAN6.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TSPAN7 antibody 18695-1-AP | Download protocol |
| IHC protocol for TSPAN7 antibody 18695-1-AP | Download protocol |
| IP protocol for TSPAN7 antibody 18695-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Macrophage lineage cells-derived migrasomes activate complement-dependent blood-brain barrier damage in cerebral amyloid angiopathy mouse model | ||
Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia | ||
J Exp Clin Cancer Res CD151-enriched migrasomes mediate hepatocellular carcinoma invasion by conditioning cancer cells and promoting angiogenesis | ||
Front Oncol TSPAN7 Exerts Anti-Tumor Effects in Bladder Cancer Through the PTEN/PI3K/AKT Pathway. | ||
Invest Ophthalmol Vis Sci De-Differentiation of Corneal Epithelial Cells Into Functional Limbal Epithelial Stem Cells After the Ablation of Innate Stem Cells | ||
Front Immunol Functional characterization of TSPAN7 as a novel indicator for immunotherapy in glioma |