• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TSH Beta Monoclonal antibody

TSH Beta Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2G1H3

Cat no : 66750-1-Ig

Synonyms

CHNG4, Thyrotropin alfa, Thyrotropin beta chain, Thyrotropin subunit beta, TSH B, TSH BETA, TSHB

Validation Data Gallery

Applications:
  • IHC staining of human pituitary using 66750-1-Ig

    IHC staining of human pituitary using 66750-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pituitary using 66750-1-Ig

    IHC staining of human pituitary using 66750-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66750-1-Ig (TSH beta antibody) at dilution of 1:6000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human pituitary using 66750-1-Ig

    IF Staining of human pituitary using 66750-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of human pituitary using 66750-1-Ig

    IF Staining of human pituitary using 66750-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human pituitary tissue using TSH Beta antibody (66750-1-Ig, Clone: 2G1H3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive IHC detected inhuman pituitary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pituitary tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

66750-1-Ig targets TSH Beta in IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen TSH Beta fusion protein Ag27153 相同性解析による交差性が予測される生物種
Full Name thyroid stimulating hormone, beta
Calculated molecular weight 138 aa, 16 kDa
GenBank accession numberBC069298
Gene symbol TSHB
Gene ID (NCBI) 7252
RRIDAB_2882097
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for TSH Beta antibody 66750-1-IgDownload protocol
IF protocol for TSH Beta antibody 66750-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols