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  • Featured Product
  • KD/KO Validated

TRPS1 Polyclonal antibody

TRPS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 20003-1-AP

Synonyms

GC79, LGCR, TRPS1, Zinc finger protein GC79

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 20003-1-AP

    WB analysis of mouse brain using 20003-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 20003-1-AP

    WB analysis of rat brain using 20003-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 20003-1-AP

    WB analysis of rat brain using 20003-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse kidney using 20003-1-AP

    WB analysis of mouse kidney using 20003-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 20003-1-AP

    WB analysis of mouse brain using 20003-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 20003-1-AP (TRPS1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa cells using 20003-1-AP

    IP experiment of HeLa cells using 20003-1-AP

    IP result of anti-TRPS1 (20003-1-AP for IP and Detection).

  • IHC staining of human prostate cancer using 20003-1-AP

    IHC staining of human prostate cancer using 20003-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

  • IHC staining of human prostate cancer using 20003-1-AP

    IHC staining of human prostate cancer using 20003-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

  • IHC staining of human kidney using 20003-1-AP

    IHC staining of human kidney using 20003-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

  • IHC staining of mouse brain using 20003-1-AP

    IHC staining of mouse brain using 20003-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 20003-1-AP

    IHC staining of mouse kidney using 20003-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 20003-1-AP

    IHC staining of mouse liver using 20003-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20003-1-AP (TRPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human kidney using 20003-1-AP

    IHC staining of human kidney using 20003-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney slide using 20003-1-AP (TRPS1 Antibody) at dilution of 1:50.

  • IF Staining of HepG2 using 20003-1-AP

    IF Staining of HepG2 using 20003-1-AP

    Immunofluorescent analysis of HepG2 cells, using TRPS1 antibody 20003-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human kidney tissue, mouse brain tissue, mouse kidney tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

20003-1-AP targets TRPS1 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name trichorhinophalangeal syndrome I
Calculated molecular weight 142 kDa
Observed molecular weight 135-142 kDa
GenBank accession numberNM_014112
Gene symbol TRPS1
Gene ID (NCBI) 7227
RRIDAB_10666999
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRPS1, also named as GC79 and LGCR, is a transcriptional repressor. TRPS1 may act to restrict expression of GATA-regulated genes at selected sites and stages in vertebrate development. It might be involved in prostate cancer apoptosis. Defects in TRPS1 are the cause of tricho-rhino-phalangeal syndrome type 1/2/3 (TRPS1) (TRPS2) (TRPS3). The antibody recognizes the C-term of TRPS1.

Protocols

Product Specific Protocols
WB protocol for TRPS1 antibody 20003-1-APDownload protocol
IHC protocol for TRPS1 antibody 20003-1-APDownload protocol
IF protocol for TRPS1 antibody 20003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Stem Cell

Multi-omic analysis reveals divergent molecular events in scarring and regenerative wound healing.

Authors - Shamik Mascharak
View Article
humanWB

J Pathol

Down-regulation of TRPS1 stimulates epithelial-mesenchymal transition and metastasis through repression of FOXA1.

Authors - Jin-Zhou Huang
  • KD Validated
View Article
humanIHC

Int J Mol Med

Pro‑differentiating compounds for human intervertebral disc cells are present in Violina pumpkin leaf extracts

Authors - Elisabetta Lambertini
View Article
WB

J Biol Chem

Dual Role of the Trps1 Transcription Factor in Dentin Mineralization.

Authors - Maria Kuzynski
  • KD Validated
View Article
humanIHC,ChIP

J Cell Physiol

Expression and function of the P2X7 receptor in human osteoblasts: The role of NFATc1 transcription factor.

Authors - Leticia Scussel Bergamin
View Article
humanIHC,IF

Life Sci

Decellularized extracellular matrix-based scaffold and hypoxic priming: A promising combination to improve the phenotype of degenerate intervertebral disc cells.

Authors - Letizia Penolazzi
View Article