Validation Data Gallery

Filter: AllWBIHC

WB analysis using 17542-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of BGC-823 using 17542-1-AP
BGC-823 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17542-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17542-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 17542-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 17542-1-AP (TRIM29 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human skin cancer using 17542-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin cancer using 17542-1-AP
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17542-1-AP (TRIM29 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skin using 17542-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skin using 17542-1-AP
Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17542-1-AP (TRIM29 Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human skin using 17542-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human skin using 17542-1-AP
Immunohistochemical analysis of paraffin-embedded human skin using 17542-1-AP (TRIM29 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

TRIM29 Polyclonal antibody

TRIM29 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 17542-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

Ataxia telangiectasia group D-associated protein, ATDC, tripartite motif containing 29, Tripartite motif-containing protein 29

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (13)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	BxPC-3 cells, A431 cells, BGC-823 cells
Positive IHC detected in	human skin cancer tissue, human skin tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:20000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 10 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 1 publications below

Product Information

17542-1-AP targets TRIM29 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag11600 Product name: Recombinant human TRIM29 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 239-588 aa of BC017352 Sequence: DQTCICYLCMFQEHKNHSTVTVEEAKAEKETELSLQKEQLQLKIIEIEDEAEKWQKEKDRIKSFTTNEKAILEQNFRDLVRDLEKQKEEVRAALEQREQDAVDQVKVIMDALDERAKVLHEDKQTREQLHSISDSVLFLQEFGALMSNYSLPPPLPTYHVLLEGEGLGQSLGNFKDDLLNVCMRHVEKMCKADLSRNFIERNHMENGGDHRYVNNYTNSFGGEWSAPDTMKRYSMYLTPKGGVRTSYQPSSPGRFTKETTQKNFNNLYGTKGNYTSRVWEYSSSIQNSDNDLPVVQGSSSFSLKGYPSLMRSQSPKAQPQTWKSGKQTMLSHYRPFYVNKGNGIGSNEAP 相同性解析による交差性が予測される生物種
Full Name	tripartite motif-containing 29
Calculated molecular weight	588 aa, 66 kDa
Observed molecular weight	65 kDa
GenBank accession number	BC017352
Gene Symbol	TRIM29
Gene ID (NCBI)	23650
RRID	AB_2272412
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14134
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TRIM29(Tripartite motif-containing protein 29) is also named as ATDC( Ataxia telangiectasia group D-associated protein). ATDC protein physically interacted with the intermediate filament protein vimentin, a protein kinase C substrate and colocalizing protein, and with an inhibitor of protein kinase C, PKCI1 (PMID:7644499). Knockdown of TRIM29 in airway epithelial cells enhances type I interferon production, and in human nasopharyngeal carcinoma cells results in almost complete Epstein-Barr virus clearance. TRIM29 is also highly induced by cytosolic double-stranded DNA in myeloid dendritic cells (PMID: 29038422). Western detected both full-length TRIM29 (65 kDa) and a truncated variant of TRIM29 with a molecular weight of about 50 kDa (PMID: 26095369).

Protocols

Product Specific Protocols
IHC protocol for TRIM29 antibody 17542-1-AP	Download protocol
WB protocol for TRIM29 antibody 17542-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse,human	WB,IP,IHC	Mol Cancer CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer. Authors - Tao Jiang KD Validated View Article
human	WB	Oncogenesis Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation. Authors - Chuan-Xia Zhang View Article
		iScience Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress Authors - Amr R Ghanam View Article
human	WB,IHC	PLoS One Ataxia-Telangiectasia Group D Complementing Gene (ATDC) Promotes Lung Cancer Cell Proliferation by Activating NF-κB Pathway. Authors - Tang Zhong-Ping ZP View Article
		Cancer Sci Role of deltaNp63(pos) CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas. Authors - Shotaro Yamano View Article
human	WB	Cancer Biol Ther iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma. Authors - Kun Wang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 17542-1-AP

WB analysis of BGC-823 using 17542-1-AP

WB analysis of A431 using 17542-1-AP

WB analysis of A431 using 17542-1-AP

WB analysis of A431 using 17542-1-AP

IHC staining of human skin cancer using 17542-1-AP

IHC staining of human skin cancer using 17542-1-AP

IHC staining of human skin using 17542-1-AP

IHC staining of human skin using 17542-1-AP

IHC staining of human skin using 17542-1-AP

IHC staining of human skin using 17542-1-AP

TRIM29 Polyclonal antibody

TRIM29 Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

CircIL4R activates the PI3K/AKT signaling pathway via the miR-761/TRIM29/PHLPP1 axis and promotes proliferation and metastasis in colorectal cancer.

Galectin-9 promotes a suppressive microenvironment in human cancer by enhancing STING degradation.

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress

Ataxia-Telangiectasia Group D Complementing Gene (ATDC) Promotes Lung Cancer Cell Proliferation by Activating NF-κB Pathway.

Role of deltaNp63(pos) CD44v(pos) cells in the development of N-nitroso-tris-chloroethylurea-induced peripheral-type mouse lung squamous cell carcinomas.

iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma.