Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of Jurkat using 12205-1-AP
WB result of TRIM27 antibody (12205-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM27 transfected Jurkat cells.
WB analysis using 12205-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 12205-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 12205-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 12205-1-AP (TRIM27 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse testis using 12205-1-AP
IP result of anti-TRIM27 (IP:12205-1-AP, 4ug; Detection:12205-1-AP 1:1000) with mouse testis tissue lysate 4000ug.
IHC staining of human lung cancer using 12205-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 12205-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12205-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 12205-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12205-1-AP (TRIM27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

TRIM27 Polyclonal antibody

TRIM27 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 12205-1-AP

Publications(22)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

EC:2.3.2.27, RFP, RNF76, TRIM, Tripartite motif-containing protein 27

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (22)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, mouse testis tissue, Raji cells
Positive IP detected in	mouse testis tissue
Positive IHC detected in	human lung cancer tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 15 publications below
IHC	See 4 publications below
IF	See 7 publications below
CoIP	See 4 publications below

Product Information

12205-1-AP targets TRIM27 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2849 Product name: Recombinant human TRIM27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 165-513 aa of BC013580 Sequence: EQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLAIYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIFAQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDNPERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWALTSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIICPMSGIDGFSGHVGNHGHSMETSP 相同性解析による交差性が予測される生物種
Full Name	tripartite motif-containing 27
Calculated molecular weight	513 aa, 58 kDa
Observed molecular weight	58 kDa, 41 kDa
GenBank accession number	BC013580
Gene Symbol	TRIM27
Gene ID (NCBI)	5987
RRID	AB_2256660
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P14373
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for TRIM27 antibody 12205-1-AP	Download protocol
IP protocol for TRIM27 antibody 12205-1-AP	Download protocol
WB protocol for TRIM27 antibody 12205-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Elife The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer. Authors - Zane A Gibbs View Article
human	CoIP	Mol Ther Nucleic Acids TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination. Authors - Li Xing View Article
human	WB	FASEB J TRIM28 and TRIM27 are required for expressions of PDGFRβ and contractile phenotypic genes by vascular smooth muscle cells. Authors - Yinfang Wang KD Validated View Article
human	WB,IF	J Cell Mol Med NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop. Authors - Bowen Hu View Article
mouse	WB	J Leukoc Biol Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes. Authors - Tongjie Wang View Article
human	WB,IF	FEBS J TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1 Authors - Juncal Garcia‐garcia KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of Jurkat using 12205-1-AP

WB analysis using 12205-1-AP

WB analysis of mouse testis using 12205-1-AP

WB analysis of Jurkat using 12205-1-AP

IP experiment of mouse testis using 12205-1-AP

IHC staining of human lung cancer using 12205-1-AP

IHC staining of human lung cancer using 12205-1-AP

IHC staining of mouse testis using 12205-1-AP

IHC staining of mouse testis using 12205-1-AP

TRIM27 Polyclonal antibody

TRIM27 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

The testis protein ZNF165 is a SMAD3 cofactor that coordinates oncogenic TGFβ signaling in triple-negative breast cancer.

TRIM27 Functions as a Novel Oncogene in Non-Triple-Negative Breast Cancer by Blocking Cellular Senescence through p21 Ubiquitination.

TRIM28 and TRIM27 are required for expressions of PDGFRβ and contractile phenotypic genes by vascular smooth muscle cells.

NT5DC2 promotes leiomyosarcoma tumour cell growth via stabilizing unpalmitoylated TEAD4 and generating a positive feedback loop.

Trim27 confers myeloid hematopoiesis competitiveness by up-regulating myeloid master genes.

TRIM27 is an autophagy substrate facilitating mitochondria clustering and mitophagy via phosphorylated TBK1