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Species Reactivity

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  • Featured Product
  • KD/KO Validated

TRIM24 Polyclonal antibody

TRIM24 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14208-1-AP

Synonyms

hTIF1, PTC6, RING finger protein 82, RNF82, TF1A, TIF1, TIF1 alpha, TIF1A, TIF1ALPHA, TRIM24, tripartite motif containing 24

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 14208-1-AP

    WB analysis of HeLa using 14208-1-AP

    WB result of TRIM24 antibody (14208-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TRIM24 transfected HeLa cells.

  • WB analysis using 14208-1-AP

    WB analysis using 14208-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of L02 using 14208-1-AP

    WB analysis of L02 using 14208-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 14208-1-AP

    WB analysis of human heart using 14208-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 14208-1-AP

    WB analysis of mouse skeletal muscle using 14208-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 14208-1-AP

    WB analysis of HeLa using 14208-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at 4 degree celsius over night.

  • WB analysis of BGC-823 using 14208-1-AP

    WB analysis of BGC-823 using 14208-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 14208-1-AP

    WB analysis using 14208-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14208-1-AP (TRIM24 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 14208-1-AP

    IP experiment of MCF-7 using 14208-1-AP

    IP result of anti-TRIM24 (IP:14208-1-AP, 4ug; Detection:14208-1-AP 1:800) with MCF-7 cells lysate 4000ug.

  • IHC staining of human prostate cancer using 14208-1-AP

    IHC staining of human prostate cancer using 14208-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with TRIM24 antibody included
  • IHC staining of human prostate cancer using 14208-1-AP

    IHC staining of human prostate cancer using 14208-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14208-1-AP (TRIM24 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with TRIM24 antibody included
  • IF Staining of HeLa using 14208-1-AP

    IF Staining of HeLa using 14208-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14208-1-AP (TRIM24 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, L02 cells, BGC-823 cells, human heart tissue, mouse skeletal muscle tissue, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TRIM24 Ready-To-Use IHC Kit. TRIM24 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14208-1-AP targets TRIM24 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRIM24 fusion protein Ag5417 相同性解析による交差性が予測される生物種
Full Name tripartite motif-containing 24
Calculated molecular weight 117 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC028689
Gene symbol TRIM24
Gene ID (NCBI) 8805
RRIDAB_2256646
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRIM24, also named as RNF82, TIF1 and TIF1A, interacts selectively in vitro with the AF2-activating domain of the estrogen receptors. Association with DNA-bound estrogen receptors, it requires the presence of estradiol. It is a regulation protein of P53. Defects in TRIM24 are a cause of thyroid papillary carcinoma (TPC) wich is a common tumor of the thyroid that typically arises as an irregular, solid or cystic mass from otherwise normal thyroid tissue.

Protocols

Product Specific Protocols
WB protocol for TRIM24 antibody 14208-1-APDownload protocol
IHC protocol for TRIM24 antibody 14208-1-APDownload protocol
IF protocol for TRIM24 antibody 14208-1-APDownload protocol
IP protocol for TRIM24 antibody 14208-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nature

TRIM24 links a non-canonical histone signature to breast cancer.

Authors - Tsai Wen-Wei WW
View Article
humanWB,CoIP

Mol Cell

p53 engages the cGAS/STING cytosolic DNA sensing pathway for tumor suppression

Authors - Monisankar Ghosh
  • KD Validated
View Article
humanWB

Mol Cell

TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.

Authors - Ai Kaiho-Soma
View Article
humanWB

Nat Chem Biol

Functional TRIM24 degrader via conjugation of ineffectual bromodomain and VHL ligands.

Authors - Lara N Gechijian
View Article
humanWB, IP, IHC

Nat Commun

TRIM24 is an oncogenic transcriptional co-activator of STAT3 in glioblastoma.

Authors - Deguan Lv
View Article
humanWB,IF

Nat Commun

Identification of proximal SUMO-dependent interactors using SUMO-ID.

Authors - Orhi Barroso-Gomila
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Parijat (Verified Customer) (09-20-2023)

Good antibody for Western

  • Applications: Western Blot