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- KD/KO Validated
TRAIP Polyclonal antibody
TRAIP Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10332-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | mouse brain tissue, A549 cells, HepG2 cells, human brain tissue, human placenta tissue, mouse placenta tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy TRAIP Ready-To-Use IHC Kit. TRAIP primary antibody included.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 7 publications below |
| IHC | See 5 publications below |
| IP | See 1 publications below |
Product Information
The immunogen of 10332-1-AP is TRAIP Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TRAIP fusion protein Ag0491 相同性解析による交差性が予測される生物種 |
| Full Name | TRAF interacting protein |
| Calculated molecular weight | 53 kDa |
| Observed molecular weight | 53 kDa |
| GenBank accession number | BC000310 |
| Gene symbol | TRAIP |
| Gene ID (NCBI) | 10293 |
| RRID | AB_10638481 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRAIP antibody 10332-1-AP | Download protocol |
| IHC protocol for TRAIP antibody 10332-1-AP | Download protocol |
| IF protocol for TRAIP antibody 10332-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci ESRG is critical to maintain the cell survival and self-renewal/pluripotency of hPSCs by collaborating with MCM2 to suppress p53 pathway | ||
Sci Rep Phycocyanin attenuates pulmonary fibrosis via the TLR2-MyD88-NF-κB signaling pathway. | ||
Biomed Pharmacother TRAIP promotes malignant behaviors and correlates with poor prognosis in liver cancer.
| ||
Int Urol Nephrol TRAIP suppressed apoptosis and cell cycle to promote prostate cancer proliferation via TRAF2-PI3K-AKT pathway activation
| ||
Cell Death Dis TRAIP modulates the IGFBP3/AKT pathway to enhance the invasion and proliferation of osteosarcoma by promoting KANK1 degradation.
| ||
Cancer Gene Ther Silencing TRAIP suppresses cell proliferation and migration/invasion of triple negative breast cancer via RB-E2F signaling and EMT
|
