TRAF7 Polyclonal antibody

TRAF7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11780-1-AP

Synonyms

RFWD1, RING finger protein 119, RNF119, TRAF7



Tested Applications

Positive WB detected inmouse heart tissue, rat heart tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11780-1-AP targets TRAF7 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TRAF7 fusion protein Ag2414 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 7
Calculated molecular weight 75 kDa
Observed molecular weight 67-75 kDa
GenBank accession numberBC024267
Gene symbol TRAF7
Gene ID (NCBI) 84231
RRIDAB_2877793
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAF7 antibody 11780-1-APDownload protocol
IHC protocol for TRAF7 antibody 11780-1-APDownload protocol
IP protocol for TRAF7 antibody 11780-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse, humanIF

Kidney Int

Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney.

Authors - Rik Westland
humanWB

Mol Ther Nucleic Acids

HFD-induced TRAF6 upregulation promotes liver cholesterol accumulation and fatty liver development via EZH2-mediated miR-429/PPARα axis.

Authors - Zhi Zhang
humanWB

Virol Sin

TRAF7 negatively regulates the RLR signaling pathway by facilitating the K48-linked ubiquitination of TBK1

Authors - Jing-Ping Huang
  • KO Validated
humanWB,CoIP

Cell Death Discov

TRAF7-targeted HOXA5 acts as a tumor suppressor in prostate cancer progression and stemness via transcriptionally activating SPRY2 and regulating MEK/ERK signaling

Authors - Jianfeng Ye
mouse,ratWB

Free Radic Biol Med

Hydrogen sulfide improves endothelial barrier function by modulating the ubiquitination degradation of KLF4 through TRAF7 S-sulfhydration in diabetic aorta

Authors - Qianzhu Li