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Species Reactivity

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated TRAF6 Monoclonal antibody

TRAF6 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

1D1E1

Cat no : CL647-66498

Synonyms

RING finger protein 85, RNF85, TRAF6

Validation Data Gallery

Applications:
  • FC experiment of Jurkat using CL647-66498

    FC experiment of Jurkat using CL647-66498

    1X10^6 Jurkat cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human TRAF6 (CL647-66498, Clone:1D1E1) (red), or 0.2 ug APC-65128 ; APC Mouse IgG2b Isotype Control (APC-65128, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy TRAF6 Ready-To-Use IHC Kit. TRAF6 primary antibody included.

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

CL647-66498 targets TRAF6 in WB, FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TRAF6 fusion protein Ag24696 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 6
Calculated molecular weight 522 aa, 60 kDa
Observed molecular weight 60 kDa
GenBank accession numberBC031052
Gene symbol TRAF6
Gene ID (NCBI) 7189
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission maxima wavelengths654 nm / 674 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.

Protocols

Product Specific Protocols
FC protocol for CL Plus 647 TRAF6 antibody CL647-66498Download protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Balkan Med J

Asiaticoside Down-Regulates HIF-1α to Inhibit Proliferation, Migration, and Angiogenesis in Thyroid Cancer Cells

Authors - Yi Zhang
View Article