Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HepG2 cells, U2OS cells |
| Positive IHC detected in | human kidney tissue, human liver tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 57 publications below |
| IHC | See 4 publications below |
| IF | See 7 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
66498-1-Ig targets TRAF6 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, fish |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24696 Product name: Recombinant human TRAF6 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-274 aa of BC031052 Sequence: MSLLNCENSCGFSQSESDCCVAMASSCSAVTKDDSVGGTASTGNLSSSFMEEIQGYDVEFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLENQLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMDCPQCQRPFQKFHINIHILKDCPRRQVSCDNCAASMAFEDKEIHDQNCPLANVICEYCNTILIREQMPNHYDLDCPTAPIPCTFSTFGCHEKMQRNHLARHLQENTQSHMRMLA 相同性解析による交差性が予測される生物種 |
| Full Name | TNF receptor-associated factor 6 |
| Calculated molecular weight | 522 aa, 60 kDa |
| Observed molecular weight | 60 kDa |
| GenBank accession number | BC031052 |
| Gene Symbol | TRAF6 |
| Gene ID (NCBI) | 7189 |
| RRID | AB_2881862 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9Y4K3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TRAF6 is a member of the TNF receptor associated factor (TRAF) protein family. TRAF6 is recognized as a signal transducer, which activates the NF-κB pathway in response to pro-inflammatory cytokines. TRAF6 has E3 ligase activity and is responsible for inducing Lys-63 (K63)-linked poly-ubiquitination chains, functioning together with E2 Ubc13/Uev1A complex to mediate IKK activation TRAF6 plays a crucial role in the regulation of the innate and adaptive immune responses.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
| IHC protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
| WB protocol for TRAF6 antibody 66498-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Mol Immunol Virus-inducible IGFALS facilitates innate immune responses by mediating IRAK1 and TRAF6 activation. | ||
Autophagy Increased alveolar epithelial TRAF6 via autophagy-dependent TRIM37 degradation mediates particulate matter-induced lung metastasis | ||
J Nanobiotechnology The ketone body β-hydroxybutyrate alleviates CoCrMo alloy particles induced osteolysis by regulating NLRP3 inflammasome and osteoclast differentiation. | ||
Theranostics A miR-146a-5p/TRAF6/NF-kB p65 axis regulates pancreatic cancer chemoresistance: functional validation and clinical significance.
| ||
Theranostics Extracellular vesicles derived from M1 macrophages deliver miR-146a-5p and miR-146b-5p to suppress trophoblast migration and invasion by targeting TRAF6 in recurrent spontaneous abortion. |