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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TRAF2 Monoclonal antibody

TRAF2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2A4D1

Cat no : 67315-1-Ig

Synonyms

TRAF2, TRAP, TRAP3

Validation Data Gallery

Applications:
  • WB analysis using 67315-1-Ig

    WB analysis using 67315-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HepG2 using 67315-1-Ig

    WB analysis of HepG2 using 67315-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 67315-1-Ig (TRAF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 67315-1-Ig

    IF Staining of HeLa using 67315-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using TRAF2 antibody (67315-1-Ig, Clone: 2A4D1 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, THP-1 cells, K-562 cells, Jurkat cells, L02 cells, HEK-293 cells
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

67315-1-Ig targets TRAF2 in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TRAF2 fusion protein Ag25387 相同性解析による交差性が予測される生物種
Full Name TNF receptor-associated factor 2
Calculated molecular weight 56 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC032410
Gene symbol TRAF2
Gene ID (NCBI) 7186
RRIDAB_2882575
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TRAF2 antibody 67315-1-IgDownload protocol
IF protocol for TRAF2 antibody 67315-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Rep

Liquidambaric acid inhibits Wnt/β-catenin signaling and colon cancer via targeting TNF receptor-associated factor 2.

Authors - Rong Yan
View Article