Validation Data Gallery

Filter: AllWBIPIHCIF-PIF/ICCFC (Intra)

WB analysis using 13343-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 13343-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 13343-1-AP (TPSAB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse liver using 13343-1-AP
IP result of anti-TPSAB1 (IP:13343-1-AP, 4ug; Detection:13343-1-AP 1:800) with mouse liver tissue lysate 4000ug.
IHC staining of human tonsillitis using 13343-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13343-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13343-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human tonsillitis using 13343-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.
IHC staining of human tonsillitis using 13343-1-AP
Immunohistochemical analysis of paraffin-embedded human tonsillitis slide using 13343-1-AP (TPSAB1 Antibody) at dilution of 1:100. Heat mediated antigen retrieved with Citric acid buffer, pH6.0.
IHC staining of human appendix using 13343-1-AP
Immunohistochemical analysis of paraffin-embedded human appendix using 13343-1-AP (TPSAB1 antibody) at dilution of 1:100 (under 10x lens).
IF Staining of mouse skin using 13343-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse skin tissue using TPSAB1 antibody (13343-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 13343-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 13343-1-AP (TPSAB1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of U-937 using 13343-1-AP
1X10^6 U-937 cells were intracellularly stained with 0.2 ug Anti-Human TPSAB1 (13343-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100.

TPSAB1 Polyclonal antibody

TPSAB1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 13343-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

EC:3.4.21.59, MCP7, TPS1, TPS2, TPSB1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (9)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	A431 cells, K-562 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human tonsillitis tissue, human appendix tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	mouse skin tissue
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	U-937 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IHC	See 4 publications below
IF	See 4 publications below

Product Information

13343-1-AP targets TPSAB1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4167 Product name: Recombinant human TPSAB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-275 aa of BC028059 Sequence: MLSLLLLALPVLASPAYAAPAPVQALQQAGIVGGQEAPRSKWPWQVSLRVRDRYWMHFCGGSLIHPQWVLTAAHCLGPDVKDLATLRVQLREQHLYYQDQLLPVSRIIVHPQFYIIQTGADIALLELEEPVNISSRVHTVMLPPASETFPPGMPCWVTGWGDVDNDEPLPPPFPLKQVKVPIMENHICDAKYHLGAYTGDDVRIIRDDMLCAGNTRRDSCQGDSGGPLVCKVNGTWLQAGVVSWDEGCAQPNRPGIYTRVTYYLDWIHHYVPKKP 相同性解析による交差性が予測される生物種
Full Name	tryptase alpha/beta 1
Calculated molecular weight	275 aa, 31 kDa
Observed molecular weight	32-38 kDa
GenBank accession number	BC028059
Gene Symbol	TPSAB1
Gene ID (NCBI)	7177
RRID	AB_2206197
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q15661
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for TPSAB1 antibody 13343-1-AP	Download protocol
IHC protocol for TPSAB1 antibody 13343-1-AP	Download protocol
IP protocol for TPSAB1 antibody 13343-1-AP	Download protocol
WB protocol for TPSAB1 antibody 13343-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Clin Transl Med Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour Authors - Kaiyuan Ji View Article
rat	IHC	Front Immunol Palmatine treats urticaria by reducing inflammation and increasing autophagy Authors - Tian Xiao View Article
human	IHC	IUBMB Life Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma. Authors - Weijuan Lou View Article
human	WB	BMC Cancer The release of tryptase from mast cells promote tumor cell metastasis via exosomes. Authors - Hui Xiao View Article
mouse,human	IHC	Am J Transl Res HucMSC-Ex alleviates DSS-induced colitis in mice by decreasing mast cell activation via the IL-33/ST2 axis Authors - Zhiping Wei View Article
human	IF	Nat Commun Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice Authors - Xiangyun Yao View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 13343-1-AP

WB analysis of A431 using 13343-1-AP

IP experiment of mouse liver using 13343-1-AP

IHC staining of human tonsillitis using 13343-1-AP

IHC staining of human tonsillitis using 13343-1-AP

IHC staining of human tonsillitis using 13343-1-AP

IHC staining of human tonsillitis using 13343-1-AP

IHC staining of human tonsillitis using 13343-1-AP

IHC staining of human appendix using 13343-1-AP

IF Staining of mouse skin using 13343-1-AP

IF Staining of HepG2 using 13343-1-AP

FC experiment of U-937 using 13343-1-AP

TPSAB1 Polyclonal antibody

TPSAB1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Integrating single-cell RNA sequencing with spatial transcriptomics reveals an immune landscape of human myometrium during labour

Palmatine treats urticaria by reducing inflammation and increasing autophagy

Bromodomain-containing protein 9 is a prognostic biomarker associated with immune infiltrates and promotes tumor malignancy through activating notch signaling pathway in negative HIF-2α clear cell renal cell carcinoma.

The release of tryptase from mast cells promote tumor cell metastasis via exosomes.

HucMSC-Ex alleviates DSS-induced colitis in mice by decreasing mast cell activation via the IL-33/ST2 axis

Dysregulated mast cell activation induced by diabetic milieu exacerbates the progression of diabetic peripheral neuropathy in mice