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  • Featured Product
  • KD/KO Validated

TPPP Polyclonal antibody

TPPP Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25040-1-AP

Synonyms

25 kDa brain specific protein, p24, p25, p25 alpha, p25alpha, TPPP, TPPP/p25, TPPP1

Validation Data Gallery

Applications:
  • WB analysis of MCF-7 using 25040-1-AP

    WB analysis of MCF-7 using 25040-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 25040-1-AP

    WB analysis using 25040-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 25040-1-AP

    WB analysis of SH-SY5Y using 25040-1-AP

    WB result of TPPP antibody (25040-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPPP transfected SH-SY5Y cells.

  • WB analysis of mouse brain using 25040-1-AP

    WB analysis of mouse brain using 25040-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 25040-1-AP (TPPP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25040-1-AP

    IHC staining of human brain using 25040-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 25040-1-AP

    IHC staining of human brain using 25040-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human breast cancer using 25040-1-AP

    IHC staining of human breast cancer using 25040-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human breast cancer using 25040-1-AP

    IHC staining of human breast cancer using 25040-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25040-1-AP (TPPP Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of MCF-7 using 25040-1-AP

    IF Staining of MCF-7 using 25040-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TPPP antibody (25040-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse brain tissue, MCF-7 cells, rat brain tissue
Positive IHC detected inhuman brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

25040-1-AP targets TPPP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPPP fusion protein Ag19159 相同性解析による交差性が予測される生物種
Full Name tubulin polymerization promoting protein
Calculated molecular weight 219 aa, 24 kDa
Observed molecular weight 24-29 kDa
GenBank accession numberBC131506
Gene symbol TPPP
Gene ID (NCBI) 11076
RRIDAB_2879865
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for TPPP antibody 25040-1-APDownload protocol
IHC protocol for TPPP antibody 25040-1-APDownload protocol
IF protocol for TPPP antibody 25040-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols