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  • Featured Product
  • KD/KO Validated

TPM3 Polyclonal antibody

TPM3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10737-1-AP

Synonyms

Gamma tropomyosin, hscp30, hTM5, NEM1, OK/SW cl.5, TM 5, TM3, TM30, TM30nm, TPM3, TPMsk3, TRK, tropomyosin 3, Tropomyosin 5, Tropomyosin alpha 3 chain

Validation Data Gallery

Applications:
  • WB analysis of mouse skeletal muscle using 10737-1-AP

    WB analysis of mouse skeletal muscle using 10737-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 10737-1-AP

    WB analysis of HepG2 using 10737-1-AP

    WB result of TPM3 antibody (10737-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TPM3 transfected HepG2 cells.

  • WB analysis using 10737-1-AP

    WB analysis using 10737-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:80000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 10737-1-AP

    WB analysis of mouse skeletal muscle using 10737-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of human heart using 10737-1-AP

    WB analysis of human heart using 10737-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse heart using 10737-1-AP

    WB analysis of mouse heart using 10737-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat heart using 10737-1-AP

    WB analysis of rat heart using 10737-1-AP

    rat heart tissue were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10737-1-AP

    WB analysis of HeLa using 10737-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 10737-1-AP

    WB analysis of HEK-293 using 10737-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10737-1-AP (TPM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 10737-1-AP

    IP experiment of HeLa using 10737-1-AP

    IP result of anti-TPM3 (IP:10737-1-AP, 4ug; Detection:10737-1-AP 1:300) with HeLa cells lysate 2000ug.

  • IHC staining of human ovary tumor using 10737-1-AP

    IHC staining of human ovary tumor using 10737-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10737-1-AP (TPM3 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 10737-1-AP

    IHC staining of human liver cancer using 10737-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

  • IHC staining of human liver cancer using 10737-1-AP

    IHC staining of human liver cancer using 10737-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

  • IHC staining of human ovary using 10737-1-AP

    IHC staining of human ovary using 10737-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

  • IHC staining of human colon cancer using 10737-1-AP

    IHC staining of human colon cancer using 10737-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

  • IHC staining of human colon cancer using 10737-1-AP

    IHC staining of human colon cancer using 10737-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

  • IHC staining of human ovary using 10737-1-AP

    IHC staining of human ovary using 10737-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary slide using 10737-1-AP (TPM3 Antibody) at dilution of 1:50.

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue, human heart tissue, HeLa cells, HEK-293 cells, rat heart tissue, HepG2 cells, rat skeletal muscle tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman ovary tumor tissue, human liver cancer tissue, human ovary tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10737-1-AP targets TPM3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TPM3 fusion protein Ag1171 相同性解析による交差性が予測される生物種
Full Name tropomyosin 3
Calculated molecular weight 33 kDa
Observed molecular weight 38-40 kDa
GenBank accession numberBC008407
Gene symbol TPM3
Gene ID (NCBI) 7170
RRIDAB_2205671
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Tropomyosins are ubiquitous proteins of 35 to 45 kDa associated with the actin filaments of myofibrils and stress fibers. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins.TPM2 subunit constitutes one part of the TPM dimer, and TPM1 or TPM3 constitutes the other part of the TPM dimer (22064019), causing an extra band of ~70kDa.

Protocols

Product Specific Protocols
WB protocol for TPM3 antibody 10737-1-APDownload protocol
IHC protocol for TPM3 antibody 10737-1-APDownload protocol
IP protocol for TPM3 antibody 10737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bone Res

Impairment of rigidity sensing caused by mutant TP53 gain of function in osteosarcoma

Authors - Ming Luo
View Article
IP

Nucleic Acids Res

Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.

Authors - Ken Asada
View Article
humanWB

Oncogene

MTBP suppresses cell migration and filopodia formation by inhibiting ACTN4.

Authors - Agarwal N N
View Article
human,mouseWB

Oncol Res

LncRNA WEE2-AS1 Knockdown Inhibits the Proliferation, Migration and 3 Invasion of Glioma Cells via Regulating miR-29b-2-5p/TPM3 Axis.

Authors - Zhen Jia
View Article
WB

J Proteome Res

Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy

Authors - Yuan Lin
View Article
humanIHC

Bioengineered

PolyC-RNA-binding protein 1 (PCBP1) enhances tropomyosin 3 (TPM3) mRNA stability to promote the progression of esophageal squamous cell carcinoma.

Authors - Kaiming Peng
View Article