Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 10942-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10942-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 10942-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 10942-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of mouse kidney using 10942-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse small intestine using 10942-1-AP
mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human small intestine using 10942-1-AP
human small intestine tissue were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 10942-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 10942-1-AP (TPK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of mouse kidney using 10942-1-AP
IP result of anti-TPK1 (IP:10942-1-AP, 4ug; Detection:10942-1-AP 1:500) with mouse kidney tissue lysate 4000ug.
IHC staining of human kidney using 10942-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10942-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10942-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10942-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 10942-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10942-1-AP (TPK1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 10942-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 10x lens).
IHC staining of human kidney using 10942-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 10942-1-AP (TPK1 Antibody) at dilution of 1:50(under 40x lens).

Featured Product
KD/KO Validated

TPK1 Polyclonal antibody

TPK1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 10942-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Thiamine pyrophosphokinase 1, Thiamin pyrophosphokinase 1, PP20, Placental protein 20, hTPK1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (10)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, HEK-293 cells, human small intestine tissue, mouse testis tissue, L02 cells, mouse small intestine tissue, mouse kidney tissue, rat small intestine tissue
Positive IP detected in	mouse kidney tissue
Positive IHC detected in	human kidney tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 10 publications below

Product Information

10942-1-AP targets TPK1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1404 Product name: Recombinant human TPK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 11-243 aa of BC014552 Sequence: LLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSIRPEVREYYATKGCELISTPDQDHTDFTKCLKMLQKKIEEKDLKVDVIVTLGGLAGRFDQIMASVNTLFQATHITPFPIIIIQEESLIYLLQPGKHRLHVDTGMEGDWCGLIPVGQPCMQVTTTGLKWNLTNDVLAFGTLVSTSNTYDGSGVVTVETDHPLLWTMAIKS 相同性解析による交差性が予測される生物種
Full Name	thiamin pyrophosphokinase 1
Calculated molecular weight	28 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC014552
Gene Symbol	TPK1
Gene ID (NCBI)	27010
RRID	AB_2205647
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H3S4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TPK1(Thiamin pyrophosphokinase 1) is also named as PP20(placental protein 20) and belongs to the thiamine pyrophosphokinase family. It is a cellular enzyme involved in the regulation of thiamine metabolism and catalyzes the conversion of thiamine, a form of vitamin B1, to thiamine pyrophosphate (TDP, or TPP). There is no difference in TPK1 expression in cultured fibroblasts from normal subjects or from patients with thiamine-responsive megaloblastic anemia(PMID:11342111). It can exsit as a dimer(PMID:21552434). Defects in TPK1 are the cause of thiamine metabolism dysfunction syndrome type 5, episodic encephalopathy type (THMD5).

Protocols

Product Specific Protocols
IHC protocol for TPK1 antibody 10942-1-AP	Download protocol
IP protocol for TPK1 antibody 10942-1-AP	Download protocol
WB protocol for TPK1 antibody 10942-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Sci Adv Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells. Authors - Rohiverth Guarecuco KO Validated View Article
mouse	WB	Proc Natl Acad Sci U S A OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin. Authors - Ligong Chen View Article
human	WB	Oncotarget Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen. Authors - Gaganpreet S Tiwana View Article
human	WB	Am J Pathol Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma. Authors - Sharma Arati A View Article
rat	WB	Sci Rep Thiamine metabolism is critical for regulating correlated growth of dendrite arbors and neuronal somata. Authors - Huimin Liu View Article
human	WB	PLoS One Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells. Authors - Shuqian Liu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 10942-1-AP

WB analysis of mouse kidney using 10942-1-AP

WB analysis of mouse testis using 10942-1-AP

WB analysis of HEK-293 using 10942-1-AP

WB analysis of mouse kidney using 10942-1-AP

WB analysis of mouse small intestine using 10942-1-AP

WB analysis of human small intestine using 10942-1-AP

WB analysis of L02 using 10942-1-AP

IP experiment of mouse kidney using 10942-1-AP

IHC staining of human kidney using 10942-1-AP

IHC staining of human kidney using 10942-1-AP

IHC staining of human colon cancer using 10942-1-AP

IHC staining of human colon cancer using 10942-1-AP

IHC staining of human colon cancer using 10942-1-AP

IHC staining of human kidney using 10942-1-AP

IHC staining of human kidney using 10942-1-AP

TPK1 Polyclonal antibody

TPK1 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Dietary thiamine influences l-asparaginase sensitivity in a subset of leukemia cells.

OCT1 is a high-capacity thiamine transporter that regulates hepatic steatosis and is a target of metformin.

Identification of vitamin B1 metabolism as a tumor-specific radiosensitizing pathway using a high-throughput colony formation screen.

Identification of aurora kinase B and Wee1-like protein kinase as downstream targets of (V600E)B-RAF in melanoma.

Thiamine metabolism is critical for regulating correlated growth of dendrite arbors and neuronal somata.

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.