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  • Featured Product
  • KD/KO Validated

p63 Monoclonal antibody

p63 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2B6B2

Cat no : 60332-1-Ig

Synonyms

AIS, B(p51A), B(p51B), CUSP, EEC3, KET, LMS, NBP, OFC8, p40, p51, p53CP, p63, p73H, p73L, RHS, SHFM4, TP53CP, TP53L, TP63, TP73L, Tumor protein 63, tumor protein p63, Tumor protein p73 like

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 60332-1-Ig

    WB analysis of HeLa using 60332-1-Ig

    WB result of TP63 antibody (60332-1-Ig, 1:1000) with si-control and si-TP63 transfected HepG2 cells.

  • WB analysis of PC-3 using 60332-1-Ig

    WB analysis of PC-3 using 60332-1-Ig

    PC-3 cells were subjected to SDS PAGE followed by western blot with 60332-1-Ig (TP63 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 60332-1-Ig

    IHC staining of human tonsillitis using 60332-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-1-Ig (TP63 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included
  • IHC staining of human tonsillitis using 60332-1-Ig

    IHC staining of human tonsillitis using 60332-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60332-1-Ig (TP63 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TP63 antibody included

Tested Applications

Positive WB detected inPC-3 cells, HeLa
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy TP63 Ready-To-Use IHC Kit. TP63 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 2 publications below

Product Information

60332-1-Ig targets p63 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen p63 fusion protein Ag2791 相同性解析による交差性が予測される生物種
Full Name tumor protein p63
Calculated molecular weight 680 aa, 77 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC039815
Gene symbol TP63
Gene ID (NCBI) 8626
RRIDAB_2881441
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Protocols

Product Specific Protocols
WB protocol for p63 antibody 60332-1-IgDownload protocol
IHC protocol for p63 antibody 60332-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Interplay and cooperation between SREBF1 and master transcription factors regulate lipid metabolism and tumor-promoting pathways in squamous cancer.

Authors - Li-Yan Li
View Article
humanIHC

Andrologia

Circ_0076305 facilitates prostate cancer development via sponging miR-411-5p and regulating PGK1.

Authors - Xiqi Ding
View Article