Validation Data Gallery

Filter: AllWBIF/ICC

WB analysis of fetal human brain using 17831-1-AP
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17831-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 17831-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17831-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 17831-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 17831-1-AP (TNPO2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IF Staining of MCF-7 using 17831-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using TNPO2 antibody (17831-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

Featured Product
KD/KO Validated

TNPO2 Polyclonal antibody

TNPO2 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 17831-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, CoIP, ELISA

Synonyms

TNPO 2, KPNB2B, Karyopherin beta-2b, IPO3, Importin 3

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	fetal human brain tissue, HEK-293 cells, HeLa cells, Jurkat cells, K-562 cells
Positive IF/ICC detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 4 publications below
IF	See 2 publications below
CoIP	See 1 publications below

Product Information

17831-1-AP targets TNPO2 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	TNPO2 fusion protein Ag12229 相同性解析による交差性が予測される生物種
Full Name	transportin 2
Calculated molecular weight	887 aa, 100 kDa
Observed molecular weight	90-100 kDa
GenBank accession number	BC072420
Gene Symbol	TNPO2
Gene ID (NCBI)	30000
RRID	AB_10598481
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14787
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for TNPO2 antibody 17831-1-AP	Download protocol
IF protocol for TNPO2 antibody 17831-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Cell Rep Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function. Authors - Eva-Maria Hock View Article
human	WB,IF	Sci Rep Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α. Authors - Tetsuji Moriyama KD Validated View Article
mouse	WB	eLife Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling Authors - Woong Y Hwang View Article
human	WB,CoIP	Int J Biol Macromol Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility Authors - Yang Liu View Article
human	WB	J Biol Chem The nuclear transportation of CHRONO regulates the circadian rhythm Authors - Qin Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of fetal human brain using 17831-1-AP

WB analysis of HeLa using 17831-1-AP

WB analysis of Jurkat using 17831-1-AP

WB analysis of HEK-293 using 17831-1-AP

WB analysis of K-562 using 17831-1-AP

IF Staining of MCF-7 using 17831-1-AP

TNPO2 Polyclonal antibody

TNPO2 Polyclonal Antibody for WB, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Hypertonic Stress Causes Cytoplasmic Translocation of Neuronal, but Not Astrocytic, FUS due to Impaired Transportin Function.

Transportin-2 plays a critical role in nucleocytoplasmic shuttling of oestrogen receptor-α.

Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling

Membrane-bound transcription factor LRRC4 inhibits glioblastoma cell motility

The nuclear transportation of CHRONO regulates the circadian rhythm