Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, mouse skeletal muscle tissue, HEK-293 cells, HeLa cells, U2OS cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 13 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
| IP | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
15104-1-AP targets TNIP1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1561 Product name: Recombinant human TNIP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 287-636 aa of BC014008 Sequence: AGKVPEVVALGAAEKKVKMLEQQRSELLEVNKQWDQHFRSMKQQYEQKITELRQKLADLQKQVTDLEAEREQKQRDFDRKLLLAKSKIEMEETDKEQLTAEAKELRQKVKYLQDQLSPLTRQREYQEKEIQRLNKALEEALSIQTPPSSPPTAFGSPEGAGALLRKQELVTQNELLKQQVKIFEEDFQRERSDRERMNEEKEELKKQVEKLQAQVTLSNAQLKAFKDEEKAREALRQQKRKAKASGERYHVEPHPEHLCGAYPYAYPPMPAMVPHHGFEDWSQIRYPPPPMAMEHPPPLPNSRLFHLPEYTWRLPCGGVRNPNQSSQVMDPPTARPTEPESPKNDREGPQ 相同性解析による交差性が予測される生物種 |
| Full Name | TNFAIP3 interacting protein 1 |
| Calculated molecular weight | 72 kDa |
| Observed molecular weight | 72 kDa |
| GenBank accession number | BC014008 |
| Gene Symbol | TNIP1 |
| Gene ID (NCBI) | 10318 |
| RRID | AB_2207757 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15025 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TNIP1, also named as KIAA0113, NAF1, Nip40-1, VAN and ABIN-1, is involved in inhibition of nuclear factor- B (NF- B) activation by interacting with TNF alpha-induced protein 3, A20 (TNFAIP3). TNIP1 repression of RARs in the presence of RA places it in a small category of corepressors of agonist-bound NRs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| IHC protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| IP protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| WB protocol for TNIP1 antibody 15104-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell TNIP1 inhibits selective autophagy via bipartite interaction with LC3/GABARAP and TAX1BP1 | ||
Cell Death Differ Ubiquitin-binding domain in ABIN1 is critical for regulating cell death and inflammation during development. | ||
BMC Biol A functional genomics screen for microRNA regulators of NF-kappaB signaling.
| ||
Retrovirology Immune regulator ABIN1 suppresses HIV-1 transcription by negatively regulating the ubiquitination of Tat.
| ||
J Cell Biochem ABIN1 inhibits HDAC1 ubiquitination and protects HDAC1 from both proteasome- and lysozyme-dependent degradation.
| ||
Clin Exp Pharmacol Physiol ABIN-1 protects chondrocytes from lipopolysaccharide-induced inflammatory injury through the inactivation of NF-κB signalling. |
