Validation Data Gallery
Tested Applications
| Positive WB detected in | HL-60 cells |
| Positive IF-P detected in | mouse heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| IHC | See 8 publications below |
| IF | See 6 publications below |
Product Information
19850-1-AP targets Thymosin beta 4 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13914 Product name: Recombinant human TMSB4X protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-63 aa of BC001631 Sequence: AQTRLRSYSCASLRFSSATMSDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 相同性解析による交差性が予測される生物種 |
| Full Name | thymosin beta 4, X-linked |
| Calculated molecular weight | 63 aa, 7 kDa |
| Observed molecular weight | 5 kDa, 10-13 kDa |
| GenBank accession number | BC001631 |
| Gene Symbol | Thymosin beta 4 |
| Gene ID (NCBI) | 7114 |
| RRID | AB_10642437 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62328 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Thymosin beta 4 (Tβ4), encoded by TMSB4X gene, is an actin sequestering protein which plays an important role in the organization of the cytoskeleton. Numerous studies have demonstrated the effects of Tβ4 on cell migration, proliferation, apoptosis and inflammation after exogenous treatment (PMID: 22652458). Recently, novel findings provided compelling evidence that Tβ4 played a key role in facilitating tumor metastasis and angiogenesis. It has been found that Tβ4 expressed increasingly in a number of metastatic tumors, thus providing a potential target of opportunity for cancer management, especially for cancer metastasis therapy (PMID: 22856429). The predicted molecular weight of the protein is 5kD, and it is reported in literature to be 10-13kDa(PMID: 8416954).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Thymosin beta 4 antibody 19850-1-AP | Download protocol |
| IHC protocol for Thymosin beta 4 antibody 19850-1-AP | Download protocol |
| WB protocol for Thymosin beta 4 antibody 19850-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Bioact Mater Sprayable bioadhesive microcarriers loaded with Tβ4-Engineered ADSC exosomes for diabetic wound healing. | ||
Nat Commun Youth-associated protein TIMP2 regulates microglial state and function in healthy and aged mice. | ||
Nat Commun Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma | ||
Acta Pharm Sin B Transient induction of actin cytoskeletal remodeling associated with dedifferentiation, proliferation, and redifferentiation stimulates cardiac regeneration | ||
Adv Sci (Weinh) Tβ4-Engineered ADSC Extracellular Vesicles Rescue Cell Senescence Through Separable Microneedle Patches for Diabetic Wound Healing | ||
Haematologica Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.
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