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  • KD/KO Validated

Thymosin beta 4 Polyclonal antibody

Thymosin beta 4 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 19850-1-AP

Synonyms

FX, PTMB4, Seraspenide, T beta 4, TB4X, THYB4, Thymosin beta 4, thymosin beta 4, X linked, Thymosin beta-4, TMSB4, TMSB4X



Tested Applications

Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inmouse skeletal muscle tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue

Recommended dilution

ApplicationDilution
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

19850-1-AP targets Thymosin beta 4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, chicken, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Thymosin beta 4 fusion protein Ag13914 相同性解析による交差性が予測される生物種
Full Name thymosin beta 4, X-linked
Calculated molecular weight 63 aa, 7 kDa
Observed molecular weight 5 kDa
GenBank accession numberBC001631
Gene symbol TMSB4X
Gene ID (NCBI) 7114
RRIDAB_10642437
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thymosin beta 4 (Tβ4), encoded by TMSB4X gene, is an actin sequestering protein which plays an important role in the organization of the cytoskeleton. Numerous studies have demonstrated the effects of Tβ4 on cell migration, proliferation, apoptosis and inflammation after exogenous treatment (PMID: 22652458). Recently, novel findings provided compelling evidence that Tβ4 played a key role in facilitating tumor metastasis and angiogenesis. It has been found that Tβ4 expressed increasingly in a number of metastatic tumors, thus providing a potential target of opportunity for cancer management, especially for cancer metastasis therapy (PMID: 22856429).

Protocols

Product Specific Protocols
IHC protocol for Thymosin beta 4 antibody 19850-1-APDownload protocol
IF protocol for Thymosin beta 4 antibody 19850-1-APDownload protocol
IP protocol for Thymosin beta 4 antibody 19850-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Commun

Single-cell transcriptomic analysis of the tumor ecosystems underlying initiation and progression of papillary thyroid carcinoma

Authors - Weilin Pu
chickenIF

Elife

Single-cell proteomics reveals changes in expression during hair-cell development.

Authors - Ying Zhu
humanWB

J Mol Cell Biol

Nanog suppresses cell migration by downregulating thymosin β4 and Rnd3.

Authors - Zhou Yizhuo Y
  • KD Validated
mouseWB

Haematologica

Thymosin β4 is essential for thrombus formation by controlling the G-actin/F-actin equilibrium in platelets.

Authors - Inga Scheller
  • KO Validated
humanIHC,IF

Cancer Gene Ther

Lysine-40 succinylation of TAGLN2 induces glioma angiogenesis and tumor growth through regulating TMSB4X

Authors - Xiaoyi Zhang
humanIHC

Sci Rep

Global Proteomics-based Identification and Validation of Thymosin Beta-4 X-Linked as a Prognostic Marker for Head and Neck Squamous Cell Carcinoma.

Authors - Li-Hsing Chi