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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

TMPO/LAP2 Monoclonal antibody

TMPO/LAP2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3G4E1

Cat no : 67157-1-Ig

Synonyms

CMD1T, LAP2, LEMD4, PRO0868, Splenin Thymopentin, thymopoietin, Thymopoietin isoform alpha, TMPO, TMPO/LAP2, TP, TP alpha, TP5, TPRP isoform alpha

Validation Data Gallery

Applications:
  • WB analysis using 67157-1-Ig

    WB analysis using 67157-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of SH-SY5Y using 67157-1-Ig

    WB analysis of SH-SY5Y using 67157-1-Ig

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HSC-T6 using 67157-1-Ig

    WB analysis of HSC-T6 using 67157-1-Ig

    HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat brain using 67157-1-Ig

    IHC staining of rat brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMPO antibody included
  • IHC staining of human liver cancer using 67157-1-Ig

    IHC staining of human liver cancer using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMPO antibody included
  • IHC staining of human liver cancer using 67157-1-Ig

    IHC staining of human liver cancer using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMPO antibody included
  • IHC staining of rat brain using 67157-1-Ig

    IHC staining of rat brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMPO antibody included
  • IHC staining of mouse brain using 67157-1-Ig

    IHC staining of mouse brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMPO antibody included
  • IHC staining of mouse brain using 67157-1-Ig

    IHC staining of mouse brain using 67157-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67157-1-Ig (TMPO/LAP2 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with TMPO antibody included
  • IF Staining of HeLa using 67157-1-Ig

    IF Staining of HeLa using 67157-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using TMPO/LAP2 antibody (67157-1-Ig, Clone: 3G4E1 ) at dilution of 1:2200 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Tested Applications

Positive WB detected inHeLa cells, SH-SY5Y cells, HSC-T6 cells, HEK-293 cells, HepG2 cells, K-562 cells
Positive IHC detected inrat brain tissue, human liver cancer tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy TMPO Ready-To-Use IHC Kit. TMPO primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67157-1-Ig targets TMPO/LAP2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity Human, rat, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen TMPO/LAP2 fusion protein Ag6415 相同性解析による交差性が予測される生物種
Full Name thymopoietin
Calculated molecular weight 51 kDa
Observed molecular weight 51 kDa, 39 kDa
GenBank accession numberBC053675
Gene symbol TMPO
Gene ID (NCBI) 7112
RRIDAB_2882454
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).

Protocols

Product Specific Protocols
WB protocol for TMPO/LAP2 antibody 67157-1-IgDownload protocol
IHC protocol for TMPO/LAP2 antibody 67157-1-IgDownload protocol
IF protocol for TMPO/LAP2 antibody 67157-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols