Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HSC-T6 cells, HeLa cells, HepG2 cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human breast cancer tissue, human liver tissue, mouse lung tissue, human liver cancer tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 8 publications below |
| IF | See 8 publications below |
Product Information
14651-1-AP targets TMPO/LAP2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, bovine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6247 Product name: Recombinant human TMPO protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-362 aa of BC053675 Sequence: MPEFLEDPSVLTKDKLKSELVANNVTLPAGEQRKDVYVQLYLQHLTARNRPPLPAGTNSKGPPDFSSDEEREPTPVLGSGAAAAGRSRAAVGRKATKKTDKPRQEDKDDLDVTELTNEDLLDQLVKYGVNPGPIVGTTRKLYEKKLLKLREQGTESRSSTPLPTISSSAENTRQNGSNDSDRYSDNEEDSKIELKLEKREPLKGRAKTPVTLKQRRVEHNQSYSQAGITETEWTSGSSKGGPLQALTRESTRGSRRTPRKRVETSEHFRIDGPVISESTPIAETIMASSNESLVVNRVTGNFKHASPILPITEFSDIPRRAPKKPLTRAEVGEKTEERRVERDILKEMFPYEASTPTGISAS 相同性解析による交差性が予測される生物種 |
| Full Name | thymopoietin |
| Calculated molecular weight | 51 kDa |
| Observed molecular weight | 75 kDa, 51 kDa and 39 kDa |
| GenBank accession number | BC053675 |
| Gene Symbol | TMPO/LAP2 |
| Gene ID (NCBI) | 7112 |
| RRID | AB_2205440 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42167 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
TMPO resides in the nucleus and may play a role in the assembly of the nuclear lamina, and thus help maintain the structural organization of the nuclear envelope. It may function as a receptor for the attachment of lamin filaments to the inner nuclear membrane. TMPO gene encodes 3 thymopoietins: alpha (75 kD), beta (51 kD), and gamma (39 kD).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| IHC protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| IP protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| WB protocol for TMPO/LAP2 antibody 14651-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol LBR and LAP2 mediate heterochromatin tethering to the nuclear periphery to preserve genome homeostasis.
| ||
Nat Cell Biol Nuclear N-glycosylation maintains H3K9me3 heterochromatin and genomic stability.
| ||
Nat Cell Biol Defective prelamin A processing promotes unconventional necroptosis driven by nuclear RIPK1 | ||
Nat Commun The missing linker between SUN5 and PMFBP1 in sperm head-tail coupling apparatus. | ||
Nat Commun RanBP2-dependent annulate lamellae drive nuclear pore assembly and nuclear expansion. | ||