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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Talin-1 Monoclonal antibody

Talin-1 Monoclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2E12D10

Cat no : 67992-1-Ig

Synonyms

ILWEQ, KIAA1027, talin 1, TLN, TLN1

Validation Data Gallery

Applications:
  • WB analysis using 67992-1-Ig

    WB analysis using 67992-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of human peripheral blood platelets using 67992-1-Ig

    WB analysis of human peripheral blood platelets using 67992-1-Ig

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 67992-1-Ig (Talin-1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67992-1-Ig

    IHC staining of human colon cancer using 67992-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 67992-1-Ig

    IHC staining of human colon cancer using 67992-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67992-1-Ig (Talin-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 67992-1-Ig

    FC experiment of HeLa using 67992-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Talin-1 (67992-1-Ig, Clone:2E12D10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inHeLa cells, human peripheral blood platelets, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 750 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

67992-1-Ig targets Talin-1 in WB, FC, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Talin-1 fusion protein Ag5937 相同性解析による交差性が予測される生物種
Full Name talin 1
Calculated molecular weight 2541 aa, 270 kDa
Observed molecular weight 270 kDa
GenBank accession numberBC042923
Gene symbol TLN1
Gene ID (NCBI) 7094
RRIDAB_2918741
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Talin-1 is a large cytoskeletal protein consisting of a globular head domain and a long rod comprised of a series of α-helical bundles (PMID: 35266014). Talin-1 is ubiquitously expressed and is a major component of focal adhesions which are multi-protein, integrin-containing complexes, mediating cell migration, adhesion and integrin signalling (PMID: 33431906). Talin-1 also plays a role in tumor formation, migration and metastasis in various malignancies (PMID: 37013489).

Protocols

Product Specific Protocols
WB protocol for Talin-1 antibody 67992-1-IgDownload protocol
IHC protocol for Talin-1 antibody 67992-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols