Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 11372-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11372-1-AP (TLE3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11372-1-AP
WB result of TLE3 antibody (11372-1-AP, 1:500) with si-Control and si-TLE3 transfected HEK293 cells.
IP experiment of HepG2 using 11372-1-AP
IP result of anti-TLE3 (IP:11372-1-AP, 4ug; Detection:11372-1-AP 1:2000) with HepG2 cells lysate 1080 ug.
IHC staining of human breast cancer using 11372-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 11372-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11372-1-AP (TLE3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 11372-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using TLE3 antibody (11372-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Featured Product
KD/KO Validated

TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11372-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Synonyms

Enhancer of split groucho-like protein 3, ESG, ESG3, GRG 3, GRG3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (23)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HeLa cells, HEK-293 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 6 publications below
WB	See 19 publications below
IHC	See 3 publications below
IF	See 5 publications below
CoIP	See 2 publications below
ChIP	See 7 publications below

Product Information

11372-1-AP targets TLE3 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1930 Product name: Recombinant human TLE3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC015729 Sequence: MYPQGRHPAPHQPGQPGFKFTVAESCDRIKDEFQFLQAQYHSLKVEYDKLANEKTEMQRHYVMYYEMSYGLNIEMHKQTEIAKRLNTILAQIMPFLSQEHQQQVAQAVERAKQVTMTELNAIIGQQQLQAQHLSHATHGPPVQLPPHPSGLQPPGIPPVTGSSSGLLALGALGSQAHLTVKDEKNHHELDHRERESSANNSVSPSESLRASEKHRGSADYSMEAKKRKAEEKDSLSRYDSDGDKSDDLVVDVSNEDPATPRVSPAHSPPENGLDKARSLKKDAPTSPASVASSSSTPSSKTKDLGHNDKSSTPGLKSNTPTPRNDAPTPGTSTTPGLRSMPGKPPGMDPI 相同性解析による交差性が予測される生物種
Full Name	transducin-like enhancer of split 3 (E(sp1) homolog, Drosophila)
Calculated molecular weight	772 aa, 83 kDa
Observed molecular weight	83 kDa
GenBank accession number	BC015729
Gene Symbol	TLE3
Gene ID (NCBI)	7090
RRID	AB_2203743
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q04726
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

TLE3, also named as KIAA1547 and ESG3, belongs to the WD repeat Groucho/TLE family. It is a transcriptional corepressor that binds to a number of transcription factors. It inhibits the transcriptional activation mediated by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. TLE3 is a member of the transducin-like enhancer of split (TLE) family of proteins that have been implicated in the tumorgenesis and classification of sarcomas. It is a candidate biomarker of response to taxane

Protocols

Product Specific Protocols
IF protocol for TLE3 antibody 11372-1-AP	Download protocol
IHC protocol for TLE3 antibody 11372-1-AP	Download protocol
IP protocol for TLE3 antibody 11372-1-AP	Download protocol
WB protocol for TLE3 antibody 11372-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,CoIP,ChIP	Nature Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer. Authors - Abhijit Parolia KD Validated View Article
mouse	WB	Cell Stem Cell Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription. Authors - Qi Li View Article
mouse	WB,ChIP	Cell Metab TLE3 is a dual-function transcriptional coregulator of adipogenesis. Authors - Villanueva Claudio J CJ View Article
mouse	WB	Cell Metab Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs. Authors - Villanueva Claudio J CJ KO Validated View Article
human	WB,chIP	J Clin Invest Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1. Authors - Leiming Wang View Article
mouse	chIP	J Exp Med Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity. Authors - Shaojun Xing KO Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 11372-1-AP

WB analysis of HEK-293 using 11372-1-AP

IP experiment of HepG2 using 11372-1-AP

IHC staining of human breast cancer using 11372-1-AP

IHC staining of human breast cancer using 11372-1-AP

IF Staining of HepG2 using 11372-1-AP

TLE3 Polyclonal antibody

TLE3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Distinct structural classes of activating FOXA1 alterations in advanced prostate cancer.

Lats1/2 Sustain Intestinal Stem Cells and Wnt Activation through TEAD-Dependent and Independent Transcription.

TLE3 is a dual-function transcriptional coregulator of adipogenesis.

Adipose subtype-selective recruitment of TLE3 or Prdm16 by PPARγ specifies lipid storage versus thermogenic gene programs.

Small molecule JQ1 promotes prostate cancer invasion via BET-independent inactivation of FOXA1.

Tle corepressors are differentially partitioned to instruct CD8+ T cell lineage choice and identity.