ZO-3 Polyclonal antibody
ZO-3 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, ELISA
Conjugate
Unconjugated
Cat no : 22116-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | MCF-7 cells, HT-29 cells, T-47D cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 2 publications below |
Product Information
The immunogen of 22116-1-AP is ZO-3 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | ZO-3 fusion protein Ag17495 相同性解析による交差性が予測される生物種 |
Full Name | tight junction protein 3 (zona occludens 3) |
Calculated molecular weight | 952 aa, 105 kDa |
Observed molecular weight | 140 kDa |
GenBank accession number | BC108906 |
Gene symbol | TJP3 |
Gene ID (NCBI) | 27134 |
RRID | AB_2878998 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for ZO-3 antibody 22116-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Free Radic Biol Med Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells. | ||
Chem Biol Interact Benzo[a]pyrene induces epithelial tight junction disruption and apoptosis via inhibiting the initiation of autophagy in intestinal porcine epithelial cells |