ZO-3 Polyclonal antibody

ZO-3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22116-1-AP

Synonyms

Tight junction protein 3, Tight junction protein ZO 3, TJP3, ZO 3, ZO3, ZO-3, Zona occludens protein 3, Zonula occludens protein 3



Tested Applications

Positive WB detected inMCF-7 cells, HT-29 cells, T-47D cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

The immunogen of 22116-1-AP is ZO-3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ZO-3 fusion protein Ag17495 相同性解析による交差性が予測される生物種
Full Name tight junction protein 3 (zona occludens 3)
Calculated molecular weight 952 aa, 105 kDa
Observed molecular weight 140 kDa
GenBank accession numberBC108906
Gene symbol TJP3
Gene ID (NCBI) 27134
RRIDAB_2878998
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for ZO-3 antibody 22116-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Free Radic Biol Med

Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.

Authors - Restituto Tocmo
pigWB

Chem Biol Interact

Benzo[a]pyrene induces epithelial tight junction disruption and apoptosis via inhibiting the initiation of autophagy in intestinal porcine epithelial cells

Authors - Jun Li