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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated TH1L Monoclonal antibody

TH1L Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1A10G2

Cat no : CL594-67380

Synonyms

HSPC130, Negative elongation factor C/D, NELF C, NELF C/D, NELF D, NELFD, TH1, TH1 like (Drosophila), TH1 like protein, TH1L

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-67380

    IF Staining of HeLa using CL594-67380

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 TH1L antibody (CL594-67380, Clone: 1A10G2 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-67380

    FC experiment of HeLa using CL594-67380

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human TH1L (CL594-67380, Clone:1A10G2) (red), or 0.8 ug CoraLite®594 Mouse IgG2a Isotype Control (C1.18.4) (CL594-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy NELFCD/TH1L Ready-To-Use IHC Kit. NELFCD/TH1L primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67380-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-67380 targets TH1L in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen TH1L fusion protein Ag24894 相同性解析による交差性が予測される生物種
Full Name TH1-like (Drosophila)
Calculated molecular weight 66 kDa
Observed molecular weight 60-66 kDa
GenBank accession numberBC014952
Gene symbol TH1L
Gene ID (NCBI) 51497
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for CL594 TH1L antibody CL594-67380Download protocol
FC protocol for CL594 TH1L antibody CL594-67380Download protocol
Standard Protocols
Click here to view our Standard Protocols